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ProteomicsProteomic analysis of S-nitrosylation in HUVECs
HUVECs stimulated with HG+oxLDL or Mannitol+nLDL were subjected to the biotin-switch assay to enrich the S-nitrosylated proteins, the S-nitrosylated proteins were resolved by SDS-PAGE and gels were silver stained. Mass spectrometry was conducted to determine proteins with S-nitrosylation.
ORGANISM(S): Homo sapiens (Human) 
2021-06-03 | PXD025295 | Pride
S-nitrosylation Oxldl Mass spectrometry Huvecs Hg 
ProteomicsFAT-switch-based quantitative S-nitrosoproteomics reveals a key role of GSNOR1 in regulating ER functions
Reversible protein S-nitrosylation regulates a wide range of biological functions and physiological activities in plants. However, it is challenging to quantitively determine the S-nitrosylation targets and dynamics in vivo. In this study, we developed a highly sensitive and efficient fluorous affin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-06-07 | PXD037504 | Pride
S-nitrosylation  fluorous affinity tags  er stress  transcription  proteomics  redox homeostasis 
TranscriptomicsGene expression data for the budding yeast mutants pol30-8, dot1Δ and pol30-8 dot1Δ
The mutation in the budding yeast gene PCNA, pol30-8, as well as deletion of DOT1 (dot1Δ), encoding the only histone H3 K79 methyltransferase in budding yeast, have been implicated in telomeric silencing. To further analyze these mutants, we used microarrays to study whether either pol30-8, dot1Δ or...
ORGANISM(S): Saccharomyces cerevisiae 
2011-04-17 | E-GEOD-27222 | biostudies-arrayexpress
GenomicsSimultaneous in vivo enzyme and host engineering through laboratory evolution
Two genetic selection systems that couple metabolite hydroxylation or methylation of small molecules to growth of Escherichia coli are presented in this study. One system targets pterin-dependent hydroxylation (tBPt) while another focuses on S-adenosylmethionine-dependent methylation (SAM). Using ad...
ORGANISM(S): Escherichia coli BW25113 
2019-01-30 | E-MTAB-6779 | biostudies-arrayexpress
MetabolomicsPlasma metabolome alteration in dairy cows with left displaced abomasum before and after surgical correction
Left displaced abomasum (LDA) leads to substantial changes in the metabolism of dairy cows. Surgical correction of LDA can rapidly improve the health of cows; however, changes in metabolism following surgery are rarely described. To investigate the changes of plasma metabolome in cows with LDA befor...
2022-08-17 | MTBLS4126 | MetaboLights
TranscriptomicsAnnotating Low Abundance and Transient RNAs in Yeast using Ultra High-throughput Sequencing
Annotating Low Abundance and Transient RNAs in Yeast using Tiling Microarrays and Ultra High-throughput Sequencing Reveals New Transcripts that are not Conserved Across Closely Related Yeast Species A complete description of the transcriptome of an organism is crucial for a comprehensive understand...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-18 | E-GEOD-11801 | biostudies-arrayexpress
UnknownMultiple immunodominant O-epitopes co-expression in live attenuated Salmonella serovars induce cross-protective immune responses against S. Paratyphi A, S. Typhimurium and S. Enteritidis.
Not available
S-EPMC9595534 | biostudies-literature
UnknownDescription of the Three Complete Mitochondrial Genomes of Sitta (S. himalayensis, S. nagaensis, and S. yunnanensis) and Phylogenetic Relationship (Aves: Sittidae).
Not available
S-EPMC10047972 | biostudies-literature
UnknownMolecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
Not available
S-EPMC4500170 | biostudies-literature
Unknown(S)-(-)-Methyl 2-(p-tolyl-sulfon-yloxy)-propanoate.
Not available
S-EPMC2961152 | biostudies-literature
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