C-terminal HA tagged hIL-33 were ectopically expressed in HEK293T cells for 24 hours, and demonstrated with indicating stimulations (Ctrl: without stimulations, Pap: 10ug/mL papain exposure for 30min, AS: 0.5mM arsenite exposure for 1hour). Cell lysates were collected for HA-immunoprecipitation and ...
Retinitis pigmentosa (RP) is an irreversible and inherited retinopathy. RPGR mutations are the most common causes of this disease. It remains challenging to decipher the mechanism of RPGR mutation because of the lack of appropriate study models. The substitution of patient-specific diseased retina w...
Here, we profiled the proteome of ten body fluids, including ascites, bile, cerebrospinal fluid (CSF), hydrothorax, knee joint fluid (KJF), plasma, saliva, serum, tear, and urine.
To understand the gain-of-toxicity of unprocessed prelamin A, we sought to identify the specific binding proteins of prelamin A that do not interact with mature lamin A. Because prelamin A is specifically localized to the nuclear envelope, we used ascorbate peroxidase 2 (APEX2)-based proximity label...
Identifying therapeutic protein targets is challenging due to unpredictable druggability. Exploring multi-purpose drug targetomes, such as that of rapamycin, offers a valuable strategy for target discovery. Here, using chemical proteomics, we identified the histone-binding protein RBBP7 as a previou...
Proteins in RAW264.7 cells were extracted by CO-IP lysis buffer, Id1 antibody and agarose gel beads were added into the lysis buffer and rotated in 4°C overnight. The agarose gel beads were washed for 5 times by centrifugation, and denatured in 2 ✖️loading buffer. The denatured protein samples were ...
