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TranscriptomicsTranscription profiling by array of the complete GlnR nitrogen-response regulon in Mycobacterium smegmatis
BACKGROUND: Nitrogen is an essential element for bacterial growth and an important component of biological macromolecules. Consequently, responding to nitrogen limitation is critical for bacterial survival and involves the interplay of signalling pathways and transcriptional regulation of nitrogen a...
ORGANISM(S): Mycobacterium smegmatis 
2013-04-24 | E-BUGS-143 | biostudies-arrayexpress
ProteomicsProteomic Time-Course Investigation into the Effects of Exposure of Mycobacterium smegmatis to Sub-Lethal Dose of Rifampicin
Drug susceptible Mycobacterium smegmatis (Mc2-155) was exposed to a sub-MIC concentration of antimycobacterial drug Rifampicin (treated) or DMSO (control). Cells were harvested at 30, 255 and 300 minutes post-exposure and the proteome at each time analysed using LC-MS/MS on a Thermo Q-Exactive.
ORGANISM(S): Mycobacterium Smegmatis Str. Mc2 155 (ncbitaxon:246196) 
2019-03-18 | MSV000083589 | MassIVE
TranscriptomicsDifferentially regulated genes induced in Mycobacterium smegmatis by in vitro acid-nitrosative multi-stress
Transcriptional profile of Mycobacterium smegmatis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MSMEG-K vs. MSMEG-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and h...
ORGANISM(S): Mycobacterium smegmatis str. MC2 155 
2013-11-05 | E-GEOD-34622 | biostudies-arrayexpress
TranscriptomicsTransciptional profile of a M. smegmatis mc2155 ΔsigF strain under standard growth conditions
Comparing transciptional profile of a M. smegmatis mc2155 ΔsigF strain and wild type (control) in in vitro culture at two time points (exponential phase and stationary phase) for identification of SigF-dependent genes. Analysis of 2 time points: exponential and stationary phase. 4 biological replic...
ORGANISM(S): Mycobacterium smegmatis 
2010-06-05 | E-GEOD-19145 | biostudies-arrayexpress
TranscriptomicsMycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))
Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to...
ORGANISM(S): Mycobacterium smegmatis 
2011-07-14 | E-GEOD-30635 | biostudies-arrayexpress
TranscriptomicsThe AmtR regulon of Mycobacterium smegmatis - Transcription profile of an M. smegmatis amtR mutant (JR258) compared to M. smegmatis mc2155 (wild-type)
To identify the AmtR regulon of Mycobacterium smegmatis, we created a markerless deletion of the amtR gene in the background of strain M. smegmatis mc2155 (wild-type) and compared the transcription profile of both strains grown in batch culture under aerobic conditions on Hartmans de Bont medium sup...
ORGANISM(S): Mycobacterium smegmatis 
2016-08-31 | E-MTAB-4857 | biostudies-arrayexpress
ProteomicsInvestigating the protein-protein interactions of EccA3 in Mycobacterium smegmatis
Mycobacterium smegmatis encodes three ESX secretion systems, ESX-1, -3 and -4. Previous research has suggested localisation of the ESX-1 secretion system at the growing polar region of M. smegmatis. In a seperate study, we demonstrated unipolar localisation of EccA3 from ESX-3 at the growing polar r...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Bacteria 
2022-06-09 | PXD018978 | Pride
Ap-ms Esx Mycobacterium smegmatis Polar localisation 
TranscriptomicsRNA-seq of wild type Mycobacterium smegmatis mc2 155 (exponential and stationary phase) and Ms1 deletion strain (exponential and stationary phase)
Ms1 RNA is ~300 nt sRNA that is highly expressed in stationary phase of growth and binds to the RNA polymerase (RNAP) core. We assume that by binding to RNAP, Ms1 could regulate transcription. Our aim was to reveal the most prominent changes in the transcriptome upon entry into stationary phase that...
ORGANISM(S): Mycolicibacterium smegmatis 
2018-11-12 | E-MTAB-7004 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Mycobacterium smegmatis response to nitrogen stress using bipartite active modules
BACKGROUND: The ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown. RESUL...
ORGANISM(S): Mycobacterium smegmatis 
2013-06-10 | E-BUGS-140 | biostudies-arrayexpress
GenomicsGenome-wide mapping of CarD, RNAPs, and RNAPb on the Mycobacterium smegmatis genome using Chromatin Immunoprecipitation Sequencing
CarD is an essential mycobacterial protein that we had previously shown to bind the RNA polymerase (RNAP) and affect the transcriptional profile of M. smegmatis and Mycobacterium tuberculosis. For this reason, we suspected that CarD was directly regulating transcriptional complexes but we did not kn...
ORGANISM(S): Mycobacterium smegmatis 
2013-08-01 | E-GEOD-48164 | biostudies-arrayexpress
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