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ProteomicsDMSO enhances electrospray response boosting sensitivity of proteomic experiments
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom up proteomics by up to tenfold. The method can be easily implemented on any ...
ORGANISM(S): Homo sapiens (Human) 
2013-08-07 | PXD000254 | Pride
Proteomics Liquid chromatography-tandem mass spectrometry Electrospray ionization Dimethyl sulfoxide 
TranscriptomicsTranscription profiling of chloride channel ClC-2 knock out mice with a Leukoencephalopathy-like phenotype vs.wild type
ClC-2 is a broadly expressed Cl- channel of the CLC family of Cl- channels and transporters which is abundantly expressed in brain. Here it was proposed to participate in lowering the cytoplasmic Cl- concentration of neurons, a process that establishes an inhibitory response to the neurotransmitters...
ORGANISM(S): Mus musculus 
2008-05-01 | E-MEXP-1028 | biostudies-arrayexpress
ProteomicsComparative proteomic and transcriptomic analysis of the impact of androgen stimulation and darolutamide inhibition on prostate cancer cells
Androgen receptor (AR) signaling is a major driver and therapy target in prostate cancer. Several inhibitors of AR function are approved for different stages of the disease and their impact on downstream gene transcription has been described. However, the ensuing effects of androgen and anti-androge...
ORGANISM(S): Homo sapiens (Human) 
2023-02-17 | PXD036962 | Pride
Andrigen receptor signalling Vcap Darolutamide 
ProteomicsIon mobility tandem mass spectrometry enhances performance of bottom-up proteomics
We describe a novel method utilizing ion mobility-based concentration of peptide fragment ions on a qTOF mass spectrometer improving the sensitivity of bottom-up proteomics by up to 10-fold. This enabled the identification of 7,500 human proteins within one day and 8,600 phosphorylation sites within...
ORGANISM(S): Homo sapiens (Human) 
2015-08-18 | PXD000863 | Pride
Quadrupole time of flight mass spectrometer Proteomics Ion mobility mass spectrometry Chemical proteomics Phosphoproteomics 
TranscriptomicsSingle cell RNA-seq of hemagglutinin specific B cells after influenza virus infection: gene expression data
Understanding dynamics of antigen specific B cell responses and link between BCR and B cell differentiation is crucial for our ability to direct immune responses and to generate memory and plasma cells responses to protective targets. Here we have infected mice with IAV-PR8 and sacrificed them at di...
ORGANISM(S): Mus musculus 
2021-06-22 | E-MTAB-9491 | biostudies-arrayexpress
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