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TranscriptomicsUbiquitous expression of long dsRNA in mice causes mainly RNAi effects in the oocyte
Double-stranded RNA (dsRNA) can enter different pathways in mammalian cells, such as the sequence-specific RNA interference (RNAi) pathway, the sequence-independent interferon response, and RNA editing by adenosine deaminases. To study routing of long dsRNA into different pathways in different tissu...
ORGANISM(S): Homo sapiens 
2011-10-10 | E-GEOD-26577 | biostudies-arrayexpress
TranscriptomicsComplexity of expression from transiently transfected plasmids
Transient plasmid transfection is common approach for studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We found that plasmids generate different levels of transcripts virtually everywhere. Spurious trans...
ORGANISM(S): Homo sapiens 
2012-08-17 | E-GEOD-36062 | biostudies-arrayexpress
TranscriptomicsExpressed dsRNA provokes selective sequence-independent PKR response in transient co-transfection experiments
In mammals, double-stranded RNA (dsRNA) plays roles in sequence-specific RNA interference, sequence-independent interferon response, and RNA editing by adenosine deaminases. We have previously shown that long hairpin dsRNA expression in cultured cells does not activate the interferon response, it is...
ORGANISM(S): Homo sapiens 
2013-12-27 | E-GEOD-46959 | biostudies-arrayexpress
TranscriptomicsA retrotransposon-driven Dicer variant enhances endogenous RNAi in mouse oocytes
Mammals have one Dicer gene required for biogenesis of small RNAs in microRNA (miRNA) and RNA interference (RNAi) pathways. Yet, endogenous RNAi is highly active in oocytes but not in somatic cells. Here, we provide a mechanistical explanation for high RNAi activity in mouse oocytes. The main Dicer ...
ORGANISM(S): Mus musculus 
2013-11-07 | E-GEOD-41207 | biostudies-arrayexpress
UnknownFunctional canonical RNAi in mice expressing a truncated Dicer isoform and long dsRNA
Not available
S-SCDT-10_1038-S44319-024-00148-Z | biostudies-other
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