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TranscriptomicsTranscription factor cooperativity in early adipogenic hotspots and super-enhancers
Here we have used a combination of advanced proteomics and genomics approaches to investigate the extent and mechanisms of transcription factor cross-talk at genomic hotspots. We identify ~12,000 transcription factor hotspots in the early phase of adipogenesis, and we find evidence of both simultane...
ORGANISM(S): Mus musculus 
2014-04-17 | E-GEOD-56872 | biostudies-arrayexpress
TranscriptomicsiRNA-seq: Computational method for genome wide assessment of acute transcriptional regulation from total RNA-seq data
RNA-seq is a sensitive and accurate technique to compare steady state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se, other technologies are needed to more precisely determine acute transcriptional responses. Here, we ha...
ORGANISM(S): Homo sapiens 
2015-02-04 | E-GEOD-60462 | biostudies-arrayexpress
UnknownBacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus.
Not available
S-EPMC7966777 | biostudies-literature
GenomicsIntegrative genomics outlines a biphasic glucose response and a ChREBP-RORγ axis regulating proliferation in β-cells
Glucose is an important regulator of pancreatic β-cell function. In addition to the acute stimulation of insulin secretion, glucose stimulates long-term adaptive changes in gene expression that can either promote or antagonize the proliferative potential and function of β-cells. The glucose-sensin...
ORGANISM(S): Rattus norvegicus 
2016-09-06 | E-GEOD-81628 | biostudies-arrayexpress
GenomicsBrowning of human adipocytes requires KLF11 and reprogramming of PPARγ super-enhancers
Here we have characterized the transcriptional processes underlying the formation of human brown in white (i.e. brite) adipocytes using a genome-wide approach. We show that the browning process is associated with reprogramming of peroxisome proliferator-activated receptor γ (PPARγ) binding to form b...
ORGANISM(S): Homo sapiens 
2014-12-12 | E-GEOD-59703 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human NIH-3T3 fibroblasts stably expressing the coxsackie-adenovirus receptor (CARI?1)) cells were transduced with adenovirus containing empty vector (AdEmpty) followed by addition of vehicle (DMSO) or transduced with adenovirus encoding either full length HA-tagged PPARg2 or A/B-domain deleted HA-PPARgCDE and treated with rosiglitazoneNIH-3T3 fibroblasts stably expressing the coxsackie-adenovirus receptor (CARI?1)) cells were transduced with adenovirus containing empty vector (AdEmpty) followed by addition of vehicle (DMSO) or transduced with adenovirus encoding either full length HA-tagged PPARg2 or A/B-domain deleted HA-PPARgCDE and treated with rosiglitazone reveals the PPARg2 A/B-domain plays a gene specific role in transactivation and co-factor recruitment
We have previously shown that adenoviral expression of peroxisome proliferator-activated receptors (PPARs) leads to rapid establishment of transcriptionally active complexes and activation of target gene expression within 5-8 h following transduction. Here we have used the adenoviral delivery system...
ORGANISM(S): Mus musculus 
2009-04-04 | E-GEOD-15433 | biostudies-arrayexpress
TranscriptomicsMolecular architecture of transcription factor hotspots in early adipogenesis
Here, we have used digital genomic footprinting to precisely define protein localization for several adipogenic transcription factors at a genome-wide level. In combination with ChIP-seq data, these analyses reveal novel molecular insight into the organization of transcription factors at hotpot regi...
ORGANISM(S): Mus musculus 
2014-05-08 | E-GEOD-57415 | biostudies-arrayexpress
GenomicsAcute TNF-induced repression of cell identity genes is mediated by NFkB-directed redistribution of cofactors from super-enhancers
The proinflammatory cytokine tumor necrosis factor (TNF) plays a central role in low-grade adipose tissue inflammation and development of insulin resistance during obesity. In this context, nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB), is directly involved and required for t...
ORGANISM(S): Homo sapiens 
2015-06-17 | E-GEOD-64233 | biostudies-arrayexpress
TranscriptomicsThe acute cold response of brown adipose tissue analyzed by RNA-seq
We applied RNA sequencing (RNA-seq) to map the global changes in gene expression of interscapular brown adipose tissue (iBAT) of mice subjected to acute cold exposure for 3 days. Here we find extensive changes in the iBAT transcriptome in response to cold with a prominent induction of genes associat...
ORGANISM(S): Mus musculus 
2015-11-25 | E-GEOD-70437 | biostudies-arrayexpress
UnknownDisruption of the acyl-CoA-binding protein gene delays hepatic adaptation to metabolic changes at weaning.
Not available
S-EPMC3030352 | biostudies-literature
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