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ProteomicsAn optimized and robust workflow for quantifying the canonical histone ubiquitination marks H2AK119ub and H2BK120ub by LC-MS/MS
The eukaryotic genome is packaged around histone proteins, which are subject to a myriad of post-translational modifications. By controlling DNA accessibility and the recruitment of protein complexes that mediate chromatin-related processes, these modifications constitute a key mechanism of epigenet...
ORGANISM(S): Homo Sapiens Mus Musculus 
2024-12-10 | PXD053212 | panorama
UnknownOptimized and Robust Workflow for Quantifying the Canonical Histone Ubiquitination Marks H2AK119ub and H2BK120ub by LC-MS/MS.
Not available
S-EPMC11932154 | biostudies-literature
ProteomicsDevelopment of a high-throughput (10 minute microflow gradient) platform for quantitation of histone modifications on a new QTOF instrument
Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The 5 histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrom...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-05-06 | MSV000094696 | MassIVE
ProteomicsDevelopment of a high-throughput (10 minute microflow gradient) platform for quantitation of histone modifications on a new QTOF instrument
Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The 5 histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrom...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-05-06 | MSV000094695 | MassIVE
GenomicsHistone methylation profiles of prospectively isolated mammary epithelial subpopulations
In this study we mapped H3K27me3, H3K4me3 and H3K9me2 marks in three mammary epithelial subsets: MaSC/basal (MS), luminal progenitor (LP) and mature luminal (ML) in the steady-state. In addition, profiles were generated for H3K4me3 and H3K27me3 marks in the MaSC/basal and luminal populations from th...
ORGANISM(S): Mus musculus 
2013-02-20 | E-GEOD-43212 | biostudies-arrayexpress
ProteomicsMultiple Reaction Monitoring assays targeting modified peptides from Plasmodium histones at 0, 18 and 36 hours post-invasion
Acid-extracted histones from the 0-hpi_2, 18-hpi_1 and 36-hpi_2 samples previously analyzed for PTMs by MudPIT were digested with endoproteinase Arg-C (RC) or endoproteinase Lys-C followed by Glu-C (KCEC). Digested histone peptides were separated on a 15cm nano-reverse phase (RP) column using a 4-hr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-06-02 | MSV000079792 | MassIVE
TranscriptomicsPhenobarbital mediates an epigenetic switch at the constitutive androstane receptor (CAR) target gene Cyp2b10 in the liver of B6C3F1 mice [MeDIP array].
Evidence suggests that epigenetic perturbations are involved in the adverse effects associated with some drugs and toxicants, including certain classes of non-genotoxic carcinogens. Such epigenetic changes (altered DNA methylation and covalent histone modifications) may take place at the earliest st...
ORGANISM(S): Mus musculus 
2011-12-16 | E-GEOD-34462 | biostudies-arrayexpress
ProteomicsAggregation of respiratory complex subunits marks the onset of proteotoxicity in proteasome inhibited cells
Proteostasis is maintained by optimum expression, folding, transport, and clearance of proteins. Deregulation of any of these processes triggers protein aggregation and is implicated in many age-related pathologies. Here, using quantitative proteomics and microscopy we show that aggregation of many ...
ORGANISM(S): Mus musculus (Mouse) 
2019-01-28 | PXD012204 | Pride
Proteomics Proteostasis Mitochondrial respiratory chain complex Low complexity region Protein turnover Mitochondria Protein misfolding Protein aggregation 
TranscriptomicsEffects of Histone H3 depletion on nucleosome occupancy and positioning through the S. cerevisiae genome [RNA_seq]
Experiments performed over the past three decades have shown that nucleosomes are transcriptional repressors. In Saccharomyces cerevisiae, depletion of histone H4 results in the genome-wide transcriptional de-repression of hundreds genes. The mechanism of de-repression is hypothesized to be rooted d...
ORGANISM(S): Saccharomyces cerevisiae 
2012-09-26 | E-GEOD-29293 | biostudies-arrayexpress
TranscriptomicsRate of elongation by RNA polymerase II is influenced by specific gene features and histone modifications
The rate of transcription elongation plays important roles in the timing of expression of full-length transcripts as well as for the regulation of alternative splicing. In this study we coupled Bru-Seq technology with 5,6-dichlorobenzimidazole 1-M-NM-2-D-ribofuranoside (DRB) to estimate the elongati...
ORGANISM(S): Homo sapiens 
2014-04-01 | E-GEOD-55534 | biostudies-arrayexpress
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