Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
Determination of the interactome of different human FLAG-HA-tagged FAM98 paralogs (FAM98A, FAM98B and FAM98C) by co-immunoprecipitation/MS. Two expression constructs were used per paralog: one with an N-terminal and one with a C-terminal FLAG-HA-tag. Expression constructs were stably integrated into...
Validation of a FAM98C KO in HEK Flp-In T-REx 293 cells by quantifying the occurrence of FAM98C-specific peptides in whole cell lysates with LC-MSMS. WT HEK Flp-In T-REx 293 cells were used as control.
This experiment showed the microarray expression of a barley recessive mutant (G132) and its wild type (Hordeum vulgare cv. Graphic) under high CO2 concentration. The homozygous mutation has a strong pleiotropic nature affecting many aspects of plant. In order to identify target genes of this mutati...
Urea-based buffers can induce carbamylation artefacts. Here we report that carbamylation of lysine residues resembles the structure of acetylated lysines (K-ac) and compete during K-ac immunoaffinity purification. We propose an alternative strategy using an ionic detergent which improves significant...
PYROXD1 is a largely unstudied essential flavoprotein, whose variants were recently reported to cause myopathies in humans. Here, we report the first biochemical and structural characterization of PYROXD1 and uncover its function as a protector of the tRNA ligase complex from oxidative inactivation....
