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To adapt SLAM-ITseq for Caenorhabditis elegans, we inserted a codon-optimized version of the Toxoplasma gondii UPRT gene downstream of a FRT-flanked mCh::his-58 cassette and under control of the strong and ubiquitous eft-3 promoter. We introduced a single copy of this construct into the C. elegans g...
ORGANISM(S): Caenorhabditis elegans 
2022-12-31 | GSE192419 | GEO
Identification of newer compounds to modulate dendritic cell functions. Total RNA obtained from bone marrow-derived dendritic cells treated for 6 hours with small chemical compounds or vehicle alone in the presence or absence of lipopolysaccharide (LPS).
ORGANISM(S): Mus musculus 
To determine the binding sites of human SCAN zinc-finger proteins (SZFPs), 293T cells transduced to express an HA-tagged SZFP in a doxycycline-inducible manner were cultured in 1 µg/ml doxycycline for 72h and were used for ChIP-seq library preparation.
ORGANISM(S): Homo sapiens 
Human HAP1 cell lines with their endogenous ZNF24 gene tagged with an FKBP12(F36V) degron were treated with dTAGv-1 to induce endogenous ZNF24 degradation to study the role of ZNF24 on histone modifications.
ORGANISM(S): Homo sapiens 
To determine the binding sites of Zscan10, mouse ES cells (mESCs) carrying Zscan10 gene endogenously tagged with 2xHA and an FKBP12(F36V) degron were treated with dTAGv-1 to induce Zscan10 degradation and were assayed with ChIPmentation against HA.
ORGANISM(S): Mus musculus 
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