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TranscriptomicsCharacterization of unique small RNA populations from rice grain
Small RNAs (~20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways in plants and animals. Here we analyze small RNA populations from mature rice grain and seedlings by pyrosequencing. Using a clustering algorithm to locate regions producing small RNAs, w...
ORGANISM(S): Oryza sativa 
2008-11-24 | E-GEOD-13152 | biostudies-arrayexpress
ProteomicsIncreased wheat protein content via introgression of a HMW glutenin selectively reshapes the grain proteome
Introgression of a high molecular weight glutenin subunit (HMW-GS) gene, 1Ay21*, into commercial wheat cultivars increased overall grain protein content and bread-making quality by unknown mechanisms. As well as increased abundance of 1Ay HMW-GS, 115 differentially expressed proteins (DEPs) were dis...
ORGANISM(S): Triticum aestivum (Wheat) 
2021-05-30 | PXD021706 | Pride
Lc-msms Mature grain Wheat 
TranscriptomicsEffect of Dietary Grain on Rumen Papillae Gene Expression in Holstein Dairy Cows
Four mature, non-lactating dairy cattle were transitioned from a high forage diet (HF; 0% grain) to a high grain diet (HG; 65% grain) that was fed for three weeks. Rumen papillae biopsies were performed during the HF baseline (week 0) and after the first (week 1) and third week (week 3) of the grai...
ORGANISM(S): Bos taurus 
2009-08-28 | E-GEOD-17849 | biostudies-arrayexpress
TranscriptomicsFunctional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation
Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has...
ORGANISM(S): Hordeum vulgare 
2011-11-22 | E-GEOD-33413 | biostudies-arrayexpress
ProteomicsCombining P and Zn fertilization to enhance yield, grain Zn availability and protein profile in maize grown on Mediterranean soils
Alkaline soils such as those found in some Mediterranean areas typically have a low phosphorus (P) and zinc (Zn) phytoavailability that detracts from plant growth and yield. We examined the effects of P and Zn fertilization individually and in combination on growth, yield and grain protein content i...
ORGANISM(S): Zea mays (Maize) 
2021-09-09 | PXD021503 | Pride
Zea mays Grain protein quality Seed storage proteins Lc-ms/ms 
ProteomicsThree Diverse Granule Preparation Methods for Proteomic Analysis of Mature Rice (Oryza sativa L.) Starch Grain
Starch is the primary form of reserve carbohydrate storage in plants. Rice (Oryza sativa L.) is a monocot whose reserve starch is organized into compounded structures within the amyloplast, rather than a simple starch grain (SG). The mechanism governing the assembly of the compound SG from polyhedra...
ORGANISM(S): Oryza sativa (Rice) 
2022-06-09 | PXD032314 | Pride
Starch grain (sg) Amyloplast Starch granule-associated protein (sgap) Septum-like structure (sls) Rice Starch granule Plastoskeleton 
ProteomicsThe Brassica Mature Pollen and Stigma Proteomes: Preparing to Meet
Successful pollination brings together the mature pollen grain and stigma papilla to initiate an intricate series of molecular processes meant to eventually enable sperm cell delivery for fertilization and reproduction. At maturity, the pollen and stigma cells have acquired proteomes comprising the...
ORGANISM(S): Brassica carinata 
2021-03-13 | PXD017953 | Pride
Proteomics Pollen germination Mass spectrometry Pollen-stigma interactions Pollen tube growth Brassica carinata Stigma Pollen Itraq Lc-ms/ms 
ProteomicsThe Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter
Triticeae crops are major contributors to global food production and ensuring their capacity to reproduce and generate seeds is critical. However, despite their importance our knowledge of the proteins underlying Triticeae reproduction is severely lacking and this is not only true of pollen and sti...
ORGANISM(S): x Triticosecale 
2023-03-08 | PXD038905 | Pride
Proteomics Brassica Mass spectrometry Pollination Triticale Triticeae Stigma Pollen Itraq 
TranscriptomicsSmall RNAs as important regulators for the hybrid vigour of super-hybrid rice
Heterosis is an important biological phenomenon; however, the role of small RNA (sRNA) in heterosis of hybrid rice remains poorly described. Here, we performed sRNA profiling of F1 super-hybrid rice LYP9 and its parents using high-throughput sequencing technology, and identified 355 distinct mature ...
ORGANISM(S): Oryza sativa 
2014-08-17 | E-GEOD-51468 | biostudies-arrayexpress
TranscriptomicsA comprehensive reference transcriptome for Brachypodium distachyon grain development incorporating key developmental transitions and identifying potential regulators
Transcriptomic analyses are particularly powerful in research when they are based on detailed underlying knowledge of developmental processes, morphological/anatomical features and biochemical/metabolic processes. Our work is based on our detailed knowledge of grain development and provides an inval...
ORGANISM(S): Brachypodium distachyon 
2019-01-31 | E-MTAB-7607 | biostudies-arrayexpress
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