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UnknownHuman DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication.
Eukaryotic genomes replicate via spatially and temporally regulated origin firing. Cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK) promote origin firing, whereas the S phase checkpoint limits firing to prevent nucleotide and RPA exhaustion. We used chemical genetics to interrogate huma...
S-EPMC8211091 | biostudies-literature
UnknownAscertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.
Brain function depends on synaptic connections between specific neuron types, yet systematic descriptions of synaptic networks and their molecular properties are not readily available. Here, we introduce SBARRO (Synaptic Barcode Analysis by Retrograde Rabies ReadOut), a method that uses single-cell ...
S-EPMC9668842 | biostudies-literature
UnknownSex-Dependent Shared and Nonshared Genetic Architecture Across Mood and Psychotic Disorders.

Background

Sex differences in incidence and/or presentation of schizophrenia (SCZ), major depressive disorder (MDD), and bipolar disorder (BIP) are pervasive. Previous evidence for shared genetic risk and sex differences in brain abnormalities across disorders suggest possible shared sex-dep...
S-EPMC8458480 | biostudies-literature
UnknownGenetic variation in human DNA replication timing.
Genomic DNA replicates in a choreographed temporal order that impacts the distribution of mutations along the genome. We show here that DNA replication timing is shaped by genetic polymorphisms that act in cis upon megabase-scale DNA segments. In genome sequences from proliferating cells, read depth...
S-EPMC4359889 | biostudies-literature
UnknownMolecular Diversity and Specializations among the Cells of the Adult Mouse Brain.
The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcription...
S-EPMC6447408 | biostudies-literature
UnknownSingle-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes.
Microglia, the resident immune cells of the brain, rapidly change states in response to their environment, but we lack molecular and functional signatures of different microglial populations. Here, we analyzed the RNA expression patterns of more than 76,000 individual microglia in mice during develo...
S-EPMC6655561 | biostudies-literature
UnknownInnovations present in the primate interneuron repertoire.
Primates and rodents, which descended from a common ancestor around 90 million years ago1, exhibit profound differences in behaviour and cognitive capacity; the cellular basis for these differences is unknown. Here we use single-nucleus RNA sequencing to profile RNA expression in 188,776 ...
S-EPMC7957574 | biostudies-literature
UnknownModeling interpretable correspondence between cell state and perturbation response with CellCap.
Single-cell transcriptomics, in conjunction with genetic and compound perturbations, offers a robust approach for exploring cellular behaviors in diverse contexts. Such experiments allow uncovering cell-state-specific responses to perturbations, a crucial aspect in unraveling the intricate molecular...
S-EPMC10979976 | biostudies-literature
TranscriptomicsImproved detection of global copy number variation using high density, non-polymorphic oligonucleotide probes
Goal: To identify copy number variation in normal individuals using high density, non-polymorphic oligonucleotide probes Background DNA sequence diversity within the human genome may be more greatly affected by copy number variations (CNVs) than single nucleotide polymorphisms (SNPs). Although the ...
ORGANISM(S): Homo sapiens 
2008-04-24 | E-GEOD-9053 | biostudies-arrayexpress
TranscriptomicsPathways Disrupted in Human ALS Motor Neurons Identified Through Genetic Correction of Mutant SOD1
Although many distinct mutations in a variety of genes are known to cause Amyotrophic Lateral Sclerosis (ALS), it remains poorly understood how they selectively impact motor neuron biology and whether they converge on common pathways to cause neural degeneration. Here, we have combined reprogramming...
ORGANISM(S): Homo sapiens 
2014-02-03 | E-GEOD-54409 | biostudies-arrayexpress
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