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Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
Plasmids encoding full-length open-reading frames (ORF) of heterogeneous nuclear ribonucleoproteins fused to a HaloTag (HT) were transfected in human HEK293T cells in biological duplicates. The expressed proteins were: HNRNPA0 (NM_006805; NP_006796.1), HNRNPA1 (NM_002136; NP_002127.1), HNRNPD (NM_00...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-04-18 | MSV000079669 | MassIVE
To identify proteins binding to Rere, we first generated a mouse colony homozygous for both the T-Cre transgene and the RS-Rere-HA allele. Thus, all the embryos generated by crossing these mice expressed Rere-HA only in mesodermal tissues. We collected around 600 E10.5 embryos and prepared whole-cel...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2017-04-13 | MSV000080920 | MassIVE
Background: Synaptic transmission is required for functional maturation of the CNS and to induce gene transcription involved in neuronal plasticity. Our aim is to identify genes that are transcriptionally regulated by synaptic transmission during development. cDNA microarrays will be used to compare...
ORGANISM(S): Drosophila melanogaster 
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