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ProteomicsA Spirodela polyrhiza genome and proteome reveal conserved chromosomal structure and translation favoring energy production
Duckweeds are a monophyletic group of rapidly reproducing aquatic monocots in the Lemnaceae family. Spirodela polyrhiza, the Greater Duckweed, has the largest body plan yet the smallest genome size in the family (1C = 150 Mb). Given their clonal, exponentially fast reproduction, a key question is wh...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-26 | PXD017093 | Pride
Arabidopsis Spyrodela Proteome composition 
ProteomicsProtein disaggregation by HSP101
Proteins that are vital to cell survival can become unfolded during heat stress, leading to the formation of toxic aggregates. Molecular chaperones, proteases and autophagic pathways are required to protect cells from the accumulation of protein aggregates. Small Heat Shock Proteins (sHSPs) form a f...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-05-14 | PXD011483 | Pride
Arabidopsis Insoluble proteins 
ProteomicsChemical damage to mRNA triggers ribosome-based quality control in the cell
Maintaining the integrity of nucleic acids is an essential feature of all organisms. Unwanted modification of DNA, if left unrepaired, is deleterious to cellular homeostasis and could have far-reaching consequences that include genomic instability and accumulation of mutations. Similarly, accumulati...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-07 | PXD013834 | Pride
Ubiquitylation Quality control Ribosome collision 
ProteomicsINTEGRATED MULTI-OMICS ANALYSES OF MAIZE REVEAL ROLES FOR AUTOPHAGIC DURING FIXED CARBON STARVATION
The turnover of cytoplasmic material via autophagic encapsulation and delivery to vacuoles is essential for recycling cellular constituents, especially under nutrient-limiting conditions. To determine how cells/tissues rely on autophagy, we applied in-depth multi-omic analyses to study maize (Zea m...
ORGANISM(S): Zea mays (Maize) 
2020-07-15 | PXD017077 | Pride
Autophagy Maize Lcms 
ProteomicsMicroautophagy of storage proteins in maize aleurone cells.
Pulldown of an ER localized HPPase to identify putative ER microautophagy receptors.
ORGANISM(S): Zea mays (Maize) 
2022-02-08 | PXD011039 | Pride
Zea mays Lc-ms Er-pulldown 
ProteomicsGenetic and proteomic screens suggest that signaling by the mechanosensitive ion channel MSL10 is influenced by its association with a synaptotagmin complex
Plants respond to mechanical stressors in a variety of ways, producing electrical and biochemical signals, altering gene expression profiles, and changing organellar organization and morphology. We used a combination of genetic and proteomic approaches to identify genes that modify signaling of MSL1...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-26 | PXD018747 | Pride
Arabidopsis Interacting proteins Mechanosensitive channels 
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