Sort   by:  
 Page size 
MetabolomicsFunctional metabolomics as a tool to analyze Mediator function and structure in plants
Mediator is a multiprotein transcriptional co-regulator complex composed of four modules; Head, Middle, Tail, and Kinase. It conveys signals from promoter-bound transcriptional regulators to RNA polymerase II and thus plays an essential role in eukaryotic gene regulation. We describe subunit localiz...
2018-01-30 | MTBLS450 | MetaboLights
MetabolomicsPseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis Mediator mutants

Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediato...

2024-02-19 | MTBLS8105 | MetaboLights
ProteomicsMolecular Architecture of the Yeast Mediator Complex
Cross-linking MS data from the yeast Mediator complex. Cross-linking was performed using either BS3 or 1:1 mix of d0:d12 DSS. Includes unfractionated, SEC enriched, high pH reverse phase fractionated, DDA and inclusion list generated files.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2015-08-13 | MSV000079237 | MassIVE
GenomicsMediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recomb...
ORGANISM(S): Homo sapiens 
2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress
TranscriptomicsMediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recomb...
ORGANISM(S): Homo sapiens 
2012-02-24 | E-GEOD-33771 | biostudies-arrayexpress
GenomicsThe Mediator complex subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination
The Mediator complex orchestrates multiple transcription factors with the Pol II apparatus for precise transcriptional control. However, its interplay with the surrounding chromatin remains poorly understood. Here, we analyze differential histone modifications between WT and MED23-/- (KO) cells and ...
ORGANISM(S): Mus musculus 
2015-09-16 | E-GEOD-59518 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis Mediator complex mutant pft1/med25 and wildtype infected with Fusarium oxysporum
The Mediator complex is an evolutionary conserved multiprotein complex that plays an essential role in initiating and regulating transcription. Its function is to act as a universal adaptor between RNA Polymerase II and DNA-bound transcription factors to translate regulatory information from activat...
ORGANISM(S): Arabidopsis thaliana 
2009-09-05 | E-GEOD-15236 | biostudies-arrayexpress
Proteomics,MultiomicsGarg_Mediator_characterization_P108_VS4
This submission contains the mass spectrometry files for the manuscript by Garg et al. that describes the identification and functional characterization of the tetrahymena mediator complex. AP-MS experiments were performed from tetrahymena thermophila cells and MS files were acquired on 5600 TripleT...
ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911) 
2019-02-26 | MSV000083486 | MassIVE
MetabolomicsMetabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival

The spread of cancer to bone is invariably fatal, with complex cross-talk between tumor cells and the bone microenvironment responsible for driving disease progression. By combining in silico analysis of patient datasets with metabolomic profiling of prostate cancer cells cultured with bone cells...

2024-08-19 | MTBLS3973 | MetaboLights
ProteomicsThe Mediator complex in the malaria parasite Plasmodium falciparum contains evolutionarily novel subunits
The eukaryote Mediator is a large and conserved multi-subunit protein complex that directly contacts RNA polymerase II and impinges on multiple aspects of gene expression. The genome of the human malaria parasite P. falciparum has been predicted to encode several Mediator subunits. We provide physic...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-05-10 | PXD027640 | Pride
Plasmodium falciparum  interactome 
Sort   by:  
 Page size