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TranscriptomicsDNA microarray analysis of genes positively regulated by HapR
Vibrio cholerae strains A1552 and ATN140 (A1552-delta-hapR) were diluted 1:100 from over night cultures and grown aerobically (at 250 rpm) in M9 minimal medium supplemented with vitamins (MEM vitamin solution, BRL) and 0.5% lactate. At OD600 ~ 0.3 2 mM (GlcNAc)6 (Seikagaku America, East Falmouth, MA...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3576 | biostudies-arrayexpress
TranscriptomicsCrab shell attachment
Strain N16961 was incubated with crab shell in artificial seawater media for 24 hours. cDNA from 1 ug RNA was labeled with Cy3 (planktonic bacteria) and Cy5 (crab attached bacteria). A growth condition experiment design type is where some part of the growth condition is changed for the purposes of t...
ORGANISM(S): Vibrio cholerae 
2005-08-11 | E-GEOD-3102 | biostudies-arrayexpress
TranscriptomicsChitin oligosaccharide induction
Strain N16961 grown in M9 + 0.5 % lactate to OD 0.2. Culture was split and one part added 0.6 mM of indicated chitin oligosaccharide. Samples was isolated after 30 and 60 min. cDNA was prepared from 2 ug RNA and labeled with Cy3 (no induction) and Cy5 (induction with chitin oligosaccharide). Samples...
ORGANISM(S): Vibrio cholerae 
2005-08-11 | E-GEOD-3101 | biostudies-arrayexpress
TranscriptomicsChitin sensor
Strains VCXB21 and VCXB21 pDel were added 0.6 mM chitobiose at early log phase. Samples isolated after 30 min. cDNA from VCXB21 was prepared from 2 ug RNA and labeled with Cy3. cDNA from VCXB21pDel was prepared from 2 ug RNA and labeled with Cy5. A genetic modification design type is where an organi...
ORGANISM(S): Vibrio cholerae 
2005-08-11 | E-GEOD-3103 | biostudies-arrayexpress
TranscriptomicsDNA microarray analysis of genes positively regulated by TfoX
Vibrio cholerae strains A1552tfoX/pBAD and A1552tfoX/pBAD-tfoX were diluted 1:100 from over night cultures and grown in LB liquid medium containing 0.2 % L-arabinose and 100 μg/ml ampicillin. At OD600=0.3, samples were quickly harvested in a microcentrifuge at room temperature and the bacterial pel...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3577 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibrio cholerae N16961 grown in M9 + 0.5 % lactate to OD 0.2, culture was split and one part added 0.6 mM of indicated chitin oligosaccharide
Strain N16961 grown in M9 + 0.5 % lactate to OD 0.2. Culture was split and one part added 0.6 mM of indicated chitin oligosaccharide. Samples was isolated after 30 and 60 min. cDNA was prepared from 2 ug RNA and labeled with Cy3 (no induction) and Cy5 (induction with chitin oligosaccharide). Samples...
ORGANISM(S): Vibrio cholerae 
2005-07-21 | E-SMDB-2237 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibrio cholerae strains VCXB21 and VCXB21 pDel with added 0.6 mM chitobiose at early log phase
Strains VCXB21 and VCXB21 pDel were added 0.6 mM chitobiose at early log phase. Samples isolated after 30 min. cDNA from VCXB21 was prepared from 2 ug RNA and labeled with Cy3. cDNA from VCXB21pDel was prepared from 2 ug RNA and labeled with Cy5.
ORGANISM(S): Vibrio cholerae 
2005-07-21 | E-SMDB-2239 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibrio cholerae strain N16961 was incubated with crab shell in artificial sea water media for 24 hours
Strain N16961 was incubated with crab shell in artificial seawater media for 24 hours. cDNA from 1 ug RNA was labeled with Cy3 (planktonic bacteria) and Cy5 (crab attached bacteria).
ORGANISM(S): Vibrio cholerae 
2005-07-21 | E-SMDB-2238 | biostudies-arrayexpress
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