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TranscriptomicsAlpha-amylase overproduction in Streptomyces lividans
The gene aml encoding alpha-amylase in Streptomyces lividans was cloned in the multicopy plasmid pIJ486, generating plasmid pAMI11. Plasmid pAMI11 and pIJ486 were propagated in S. lividans TK21 to obtain S. lividans TK21(pAMI11) and its isogenic strain S. lividans TK21(pIJ486). Transcriptional profi...
ORGANISM(S): Streptomyces lividans 
2015-07-23 | E-GEOD-61448 | biostudies-arrayexpress
TranscriptomicsAgarase overproduction in Streptomyces lividans
The gene dagA encoding agarase in Streptomyces coelicolor was cloned in the multicopy plasmid pIJ486 generating plasmid pAGAs5. Plasmid pAGAs5 and pIJ486 were propagated in S. lividans TK21 to obtain S. lividans TK21(pAGAs5) and its isogenic strain S. lividans TK21(pIJ486). Transcriptional profiling...
ORGANISM(S): Streptomyces lividans 
2015-07-23 | E-GEOD-61447 | biostudies-arrayexpress
TranscriptomicsThe Streptomyces coelicolor DegS-DegU-like regulon
The Streptomyces coelicolor two genes operon SCO5784-SCO5785 encodes a two-component system which functions in a similar manner to that of the Bacillus subtilis DegS-DegU system. Propagation of the regulatory gene in high copy number results in the overproduction of several extracellular enzymes, am...
ORGANISM(S): Streptomyces coelicolor 
2012-05-03 | E-GEOD-23195 | biostudies-arrayexpress
TranscriptomicsTranslocase malfunction causes extracellular protein translocation stress ( EPTS) in Streptomyces lividans
The genomic and proteomic analyses of Streptomyces lividans strains deficient in the major signal peptidase SipY or in the translocase complex protein SecG resulted in a set of genes being equally regulated. These genes are apparently responsible for the common deficiencies in extracellular protein ...
ORGANISM(S): Streptomyces lividans 
2012-05-03 | E-GEOD-17012 | biostudies-arrayexpress
TranscriptomicsThe Streptomyces lividans lsp mutant
The Streptomyces lividans lsp gene encodes a type II signal peptidase (Lsp) that cleaves the type II leader peptides of lipoproteins. Transcriptional profiling of the bacterium depleted of the lsp gene mainly resulted in deactivation of the sigma U regulon, as well as in downregulation of genes invo...
ORGANISM(S): Streptomyces lividans 
2014-05-22 | E-GEOD-30728 | biostudies-arrayexpress
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