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MetabolomicsDifferent regions of synaptic vesicle membrane regulate VAMP2 conformation for the SNARE assembly
Vesicle associated membrane protein 2 (VAMP2/synaptobrevin2), a core SNARE protein residing on synaptic vesicles (SVs), forms helix bundles with syntaxin-1 and SNAP25 for the SNARE assembly. Prior to the SNARE assembly, the structure of VAMP2 is unclear. Here, by using in-cell NMR spectroscopy, we d...
2021-10-06 | MTBLS1503 | MetaboLights
MetabolomicsLION-web validation lipidomics experiments (FA-incorporation, membrane fluidty)
A major challenge for lipidomic analyses is the handling of the large amounts of data and the translation of results to interpret the involvement of lipids in biological systems. We built a new lipid ontology (LION) that associates over 50,000 lipid species to biophysical, chemical and cell biologic...
2019-04-24 | MTBLS757 | MetaboLights
MetabolomicsControlling mitochondrial membrane architecture via MIC60 determines viral replication to promote anti-viral immunity
Virus-infected cells often exhibit dramatic cellular changes accompanied by altered mitochondrial function. The contribution of factors shaping the inner mitochondrial membrane (IMM) and cristae architecture on viral replication is insufficiently understood. Single cell transcriptomics applying vesi...
2025-06-24 | MTBLS12437 | MetaboLights
ProteomicsPlasma and crude membrane fraction from mouse liver and kidney
The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions ...
ORGANISM(S): Mus Musculus (mouse) 
2020-09-26 | PXD018161 | JPOST Repository
TranscriptomicsCotranslational signal independent SRP preloading during membrane targeting
Cotranslational targeting into the endoplasmic reticulum (ER) by the Signal Recognition Particle (SRP) is a key event determining polypeptide fate in eukaryotic cells. Here, we globally define the principles and mechanisms of SRP binding and ER targeting in vivo. Cotranslational targeting through SR...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-03 | E-GEOD-74393 | biostudies-arrayexpress
TranscriptomicsMembrane-polysome associated fractionation of HES2 embryonic stem cells
The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA Three consecutive passages of HES2 cells were grown and subject to membrane-polysome fractionation....
ORGANISM(S): Homo sapiens 
2010-04-07 | E-GEOD-14037 | biostudies-arrayexpress
TranscriptomicsSecreted and membrane-associated genes
Large-scale identification of secreted and membrane-associated gene products using DNA microarrays. This study is described more fully in Diehn M et al.(2000) Nat Genet 25:58-62
ORGANISM(S): Homo sapiens 
2002-06-27 | E-GEOD-48 | biostudies-arrayexpress
TranscriptomicsGenome-Scale Identification of Membrane-Associated Human mRNAs
The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficie...
ORGANISM(S): Homo sapiens 
2006-02-24 | E-GEOD-4175 | biostudies-arrayexpress
GenomicsMembrane fraction RNA-seq of active and dormant (ovoid) M. smegmatis
Membrane fraction RNA-seq of active and dormant (ovoid) M. smegmatis
PRJNA837108 | ENA
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
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