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TranscriptomicsMicroRNA profiling of primary cutaneous large B-cell lymphomas
High-throughput sequencing of primary cutaneous follicle center lymphoma (PCFCL), primary cutaneous diffuse large B-cell lymphoma, leg type (PCLBCL-LT) and in vitro activated peripheral blood B-cells. We performed high-throughput sequencing analysis on frozen tumor biopsies from 19 cases of PCFCL an...
ORGANISM(S): Homo sapiens 
2013-10-18 | E-GEOD-51359 | biostudies-arrayexpress
TranscriptomicsTranscriptome Profiling of Developing Murine Lens through RNA Sequencing
Purpose: Transcriptome is the entire repertoire of all transcripts present in a cell at any particular time. We undertook next-generation whole transcriptome sequencing approach to gain insight of the transcriptional landscape of the developing mouse lens. Methods: We ascertained mice lenses at six ...
ORGANISM(S): Mus musculus 
2015-10-29 | E-GEOD-69221 | biostudies-arrayexpress
TranscriptomicsMicroRNA sequence and expression analysis in breast tumors by deep sequencing
This SuperSeries is composed of the following subset Series: GSE29173: MicroRNA sequence and expression analysis in breast tumors by deep sequencing [miRNA sequence data] GSE29174: MicroRNA sequence and expression analysis in breast tumors by deep sequencing [mRNA expression array data] MicroRNAs (m...
ORGANISM(S): Homo sapiens 
2011-06-07 | E-GEOD-28884 | biostudies-arrayexpress
TranscriptomicsDerivation of Rhesus Monkey Parthenogenetic Embryonic Stem Cells and its MicroRNA Signature
We derived two novel rpESC lines and characterized their microRNA signature by Solexa deep sequencing. By characterizing their microRNA signature, we identified 91 novel microRNAs, except those are also detected in other primate ESCs. Moreover, these two novel rpESCs display a unique microRNA signat...
ORGANISM(S): Macaca mulatta 
2011-10-18 | E-GEOD-30510 | biostudies-arrayexpress
TranscriptomicsDeep sequencing of microRNA present in ejaculated bull spermatozoa
The objectives of our study were to identify microRNA (miRNA) present in bovine sperm. Angus bulls (n=6) were assigned to treatments of either toxic or non-toxic fescue seed diets. Semen was collected and subjected to RNA isolation, enriched for miRNA. Three samples from each treatment group were ch...
ORGANISM(S): Bos taurus 
2014-12-10 | E-GEOD-61746 | biostudies-arrayexpress
TranscriptomicsIntegrated analysis of microRNA and mRNA expression and association with HIF binding in MCF-7 cells under hypoxia (miRNA-Seq)
Purpose: We aimed to investigate in depth the regulation of microRNA expression by hypoxia in the breast cancer cell line MCF-7, establish the relationship between microRNA expression and HIF binding sites, pri-miRNA transcription and microRNA processing gene expression. Methods: microRNA sequencing...
ORGANISM(S): Homo sapiens 
2014-02-13 | E-GEOD-47602 | biostudies-arrayexpress
TranscriptomicsYM500: An integrative small RNA sequencing (smRNA-seq) database for microRNA research
MicroRNAs (miRNAs) are small RNAs of ~22 nucleotides in length that are involved in the regulation of a variety of physiological and pathological processes. Advances in high-throughput small RNA sequencing (smRNA-seq), one of the next generation sequencing applications, have reshaped the miRNA resea...
ORGANISM(S): Homo sapiens 
2012-12-03 | E-GEOD-39841 | biostudies-arrayexpress
TranscriptomicsNext-Generation Sequencing of Small RNAs from HIV-Infected Cells Identifies Phased microRNA Expression Patterns and Candidate Novel microRNAs Differentially Expressed upon Infection
HIV infection of CD4 T cells induces a range of host transcriptional changes in mRNAs as well as microRNAs that may coordinate changes in mRNAs. We applied next-generation sequencing, analyzing the small RNA fraction of HIV-infected cells at 5, 12, and 24 h postinfection (RNA-Seq).
ORGANISM(S): Homo sapiens 
2014-05-17 | E-GEOD-57763 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Small RNA
Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in seve...
ORGANISM(S): Mus musculus 
2014-03-12 | E-GEOD-55791 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Small RNA, after 1 week pressure overload
Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. 5 female mice were subjected to a sham operation, and 5 female mice were subjected to transv...
ORGANISM(S): Mus musculus 
2014-04-18 | E-GEOD-56891 | biostudies-arrayexpress
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