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The aim of the present study was to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at proteome level. The PEG-induced osmotic stress was studied in cotyledonary MDE of two genotypes: Cadeli (D), and Viking (V), which were previously reported to reveal contras...
ORGANISM(S): Brassica napus (Rape) 
2021-06-14 | PXD024552 | Pride
Transcriptome profiling of heat-induced tobacco microspore reprogramming and embryogenesis
SHOOTMERISTEMLESS (STM) is a well characterized key regulator of the shoot apical meristem (SAM) activity in diverse plant species. We used a transgenic approach to investigate how the altered expression of Brassica napus (Bn) STM affects the morphology and behavior of microspore-derived embryos (M...
ORGANISM(S): Brassica napus 
The transcriptional landscape of microspore embryogenesis induction in barley
Four cDNA libraries from immature embryos, mature embryos, microspore derived embryos and mature leaves were constructed. cDNA were sequenced by the Roche-454 GS-FLX.
ORGANISM(S): Cocos nucifera 
The effectiveness of microspore embryogenesis and plant formation from in vitro cultivated microspores is determined by a complex network of internal and environmental factors. Plant breeding and genetic engineering widely use haploids/doubled haploids (DHs) derived from in vitro-cultured microspore...
ORGANISM(S): Hordeum vulgare 
2024-12-29 | GSE233486 | GEO
Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana) and two restorers ones (Patres and Primépi) were used to Representational Difference Analysis (cDNA-RDA) to identify effective Rf (fertility restorer) genes. Anthers at the microspore stage were used as testers while ant...
ORGANISM(S): Triticum aestivum 
Small RNA diversity and function has been widely characterized in various tissues of the sporophytic generation of the angiosperm model Arabidopsis thaliana. In contrast, there is limited knowledge about small RNA diversity and their roles in developing male gametophytes. We thus carried out small R...
ORGANISM(S): Arabidopsis thaliana 
The few-celled microspore embryo transcriptome of Brassica napus
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