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UnknownMicrotubule-binding-induced allostery triggers LIS1 dissociation from dynein prior to cargo transport.
The lissencephaly-related protein LIS1 is a critical regulator of cytoplasmic dynein that governs motor function and intracellular localization (for example, to microtubule plus-ends). Although LIS1 binding is required for dynein activity, its unbinding prior to initiation of cargo transport is equa...
S-EPMC10590275 | biostudies-literature
UnknownSingle-molecule microtubule dynamics measurements reveal an intermediate state and clarify the role of nucleotide.
Microtubules are polymers of αβ-tubulin heterodimers that undergo GTPase dependent polymerization dynamics to organize the intracellular space and mediate faithful chromosome segregation. Microtubule dynamics derive from biochemical properties of individual tubulin subunits and how they interact wit...
S-EPMC12808227 | biostudies-literature
UnknownIn Silico Exploration of Microtubule Agent Griseofulvin and Its Derivatives Interactions with Different Human β-Tubulin Isotypes.
Tubulin isotypes are known to regulate microtubule stability and dynamics, as well as to play a role in the development of resistance to microtubule-targeted cancer drugs. Griseofulvin is known to disrupt cell microtubule dynamics and cause cell death in cancer cells through binding to tubulin prote...
S-EPMC10005519 | biostudies-literature
UnknownInteraction of microtubule depolymerizing agent indanocine with different human αβ tubulin isotypes.
Tubulin isotypes are known to regulate the stability and dynamics of microtubules, and are also involved in the development of resistance against microtubule-targeted cancer drugs. Indanocine, a potent microtubule depolymerizing agent, is highly active against multidrug-resistant (MDR) cancer cells ...
S-EPMC5870988 | biostudies-literature
UnknownQuantification of Engagement of Microtubules by Small Molecules in Living Cells by Flow Cytometry.
Drugs such as paclitaxel (Taxol) that bind microtubules are widely used for the treatment of cancer. Measurements of the affinity and selectivity of these compounds for their targets are largely based on studies of purified proteins, and only a few quantitative methods for the analysis of interactio...
S-EPMC9585582 | biostudies-literature
UnknownThe XMAP215 family drives microtubule polymerization using a structurally diverse TOG array.
XMAP215 family members are potent microtubule (MT) polymerases, with mutants displaying reduced MT growth rates and aberrant spindle morphologies. XMAP215 proteins contain arrayed tumor overexpressed gene (TOG) domains that bind tubulin. Whether these TOG domains are architecturally equivalent is un...
S-EPMC4142611 | biostudies-literature
UnknownKinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs.

Background

Kinesin-3 family motors drive diverse cellular processes and have significant clinical importance. The ATPase cycle is integral to the processive motility of kinesin motors to drive long-distance intracellular transport. Our previous work has demonstrated that kinesin-3 motors are...
S-EPMC9364601 | biostudies-literature
UnknownThe C terminus of tubulin, a versatile partner for cationic molecules: binding of Tau, polyamines, and calcium.
The C-terminal region of tubulin is involved in multiple aspects of the regulation of microtubule assembly. To elucidate the molecular mechanisms of this regulation, we study here, using different approaches, the interaction of Tau, spermine, and calcium, three representative partners of the tubulin...
S-EPMC3024800 | biostudies-literature
UnknownExploring the Origin of Differential Binding Affinities of Human Tubulin Isotypes αβII, αβIII and αβIV for DAMA-Colchicine Using Homology Modelling, Molecular Docking and Molecular Dynamics Simulations.
Tubulin isotypes are found to play an important role in regulating microtubule dynamics. The isotype composition is also thought to contribute in the development of drug resistance as tubulin isotypes show differential binding affinities for various anti-cancer agents. Tubulin isotypes αβII, αβIII a...
S-EPMC4882049 | biostudies-literature
UnknownA communication hub for phosphoregulation of kinetochore-microtubule attachment.
The Mps1 and Aurora B kinases regulate and monitor kinetochore attachment to spindle microtubules during cell division, ultimately ensuring accurate chromosome segregation. In yeast, the critical spindle attachment components are the Ndc80 and Dam1 complexes (Ndc80c and DASH/Dam1c, respectively). Nd...
S-EPMC11847324 | biostudies-literature
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