We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We perfor...
In E. coli, the pACYC-HA-Ub-E1 (UBA1)-UbcH5A-RNF126 and pET-22b-MIDN-His₆ plasmids were co-transformed. After IPTG induction to express all proteins required for ubiquitination, His-tagged MIDN was enriched with Ni-NTA resin and subjected to mass-spectrometric mapping of its ubiquitin-modified sites...
This study investigates the role of Midnolin (Midn) in regulating hepatic transcriptional and chromatin landscapes in vivo. Midn is a ubiquitin-like domain–containing nuclear protein that mediates ubiquitin-independent proteasomal degradation of transcription factors. Although Midn has been implicat...
targeted associated bisulfite sequencing (TaBA-seq) on LINE1 elements in Midnolin (Midn) KO ESCs, and wild-type (wt) ESCs harboring a doxycycline-inducible Midnolin transgene in which Midnolin overexpression was induced for 3 days
We identified the essential gene encoding midnolin in a genetic screen in which multiple Midn alleles caused reductions in peripheral B cells and T cell-independent antibody responses. Causation was confirmed in mice with targeted deletion of 4 of 6 MIDN protein isoforms. MIDN augmented proteasome a...
