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ProteomicsRole of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit
In this work, we show that 23S rRNA lacking the SRL can be efficiently reconstituted into 50S subunits, which exhibit full peptidyl transferase (PT) activity. Using cryo-EM and proteomic methods, we also further characterize affinity-purified SRL particles isolated from cells. Collectively, these da...
ORGANISM(S): Escherichia coli 
2025-05-07 | PXD056260 | Pride
Lc-msms Escherichia coli 50s ribosomal subunit Proteomics 
ProteomicsIntegrated systems analysis reveals conserved gene networks underlying response to spinal cord injury
Spinal cord injury (SCI) is a devastating neurological condition for which there are currently no effective treatment options to restore function. A major obstacle to the development of new therapies is our fragmentary understanding of the coordinated pathophysiological processeses triggered by dama...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-09-25 | PXD010192 | Pride
Rat Spinal cord injury 
ProteomicsTrypanosoma cruzi, oral infection, cruzipain, gp85/Trans- sialidase family
Oral transmission of T. cruzi is probably the most frequent mechanism among animals in the wild. In this context, there is a high prevalence of human infections in regions where triatomine infection with T. cruzi is low. This led to the hypothesis that the consumption of raw or undercooked meat from...
ORGANISM(S): Trypanosoma cruzi Homo sapiens (Human) Anisakis simplex 
2024-05-21 | PXD044267 | Pride
Gp85/trans- sialidase family Cruzipain Oral infection Trypanosoma cruzi 
ProteomicsComprehensive proteomic analysis of autophagosomes derived from Leishmania infected macrophages
Autophagy has been implicated as a host defense mechanism against intracellular pathogens. However, certain intracellular pathogens such as Leishmania can manipulate the host’s autophagy to promote their survival. Recently, our findings regarding the regulation of autophagy by Leishmania donovani in...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037057 | Pride
Parasitic infection Autophagy Autophagosome Leishmania donovani Host-pathogen interactions Lcmsms 
ProteomicsCharacterization of Argonaute-containing protein complexes in Leishmania-infected human macrophages
Leishmania donovani, an intracellular protozoan parasite, is the causative agent of visceral leishmaniasis or kala-azar, the most severe form of leishmaniasis in humans. To date, our understanding of the molecular mechanisms associated with the pathogenicity of Leishmania infection is still limited....
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD037042 | Pride
Leishmania Rnai Macrophage Lcmsms 
ProteomicsQuantitative proteomic screen identifies Annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2
In this work we use stable isotope labeling of amino acids in cell culture (SILAC) and a S. Typhimurium mutant that secretes increased amounts of effectors to identify cognate effector binding partners during infection. Using this method, we identified the host protein annexin A2 as a binding partne...
ORGANISM(S): Homo sapiens (Human) Salmonella enterica subsp. enterica serovar Typhimurium str. 5579 Salmonella enterica subsp. enterica serovar Typhimurium str. A130 
2022-02-17 | PXD025582 | Pride
Proteomics Silac Salmonella 
ProteomicsLeishmania regulates host YY1: comparative proteomic analysis identifies infection modulated YY1 dependent proteinsLeishmania regulates host YY1: comparative proteomic analysis identifies infection modulated YY1 dependent proteins
The protein Yin-Yang 1 (YY1) is a ubiquitous multifunctional transcription factor. Interestingly, there are several cellular functions controlled by YY1 that could play a role in Leishmania pathogenesis. Leishmaniasis is a human disease caused by protozoan parasites of the genus Leishmania. This stu...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD059968 | Pride
Parasitic infection Leishmania Transcription factor Proteomics Host-pathogen interactions Lcmsms 
ProteomicsThe life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera)
Deleterious mutations arise continually in natural populations, yet their full life histories—from origin to elimination—are rarely observed directly. Most understanding of mutational fate in the wild comes from indirect inference rather than from tracking a single mutation across generations. Haplo...
ORGANISM(S): Apis mellifera (Honeybee) 
2026-09-07 | PXD075256 | Pride
Eye mutation Ivory mutation Cinnabar Hymenoptera Genome-wide association study Recurrent mutation 
ProteomicsConserved GTPase LepA (Elongation Factor 4) functions in biogenesis of the 30S subunit of the 70S ribosome
The physiological role of LepA, a paralog of EF-G found in all bacteria, has been a mystery for decades. Here, we show that LepA functions in ribosome biogenesis. In cells lacking LepA, immature 30S particles accumulate. Four proteins are specifically underrepresented in these particles-S3, S10, S14...
ORGANISM(S): Escherichia coli 
2019-07-03 | PXD014436 | Pride
Ef4  rsga  yjeq  protein synthesis  translation 
ProteomicsLeishmania exosomal tRNA-derived small non-coding RNA fragments modulate host macrophage proteins: A quantitative proteomic analysis
Protozoan parasites of the genus Leishmania, the causative agents of human leishmaniasis, have evolved mechanisms to manipulate host cell functions to their advantage. Recently, small non-coding RNAs have been identified as contributors to the pathogenesis of Leishmania infection. An increasing numb...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD072695 | Pride
Parasitic infection And proteomics Leishmaniasis Leishmania Macrophages Trna fragments Host-pathogen interactions 
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