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TranscriptomicsSpecies-specific factors mediate extensive heterogeneity of mRNA 3' ends in yeasts
Most eukaryotic genes express mRNAs with alternative polyadenylation sites at their 3’ ends. Here we show that polyadenylated 3’ termini in three yeast species (S. cerevisiae, K. lactis, D. hansenii) are remarkably heterogeneous. Instead of a few discrete 3’ ends, the average yeast gene has an “en...
ORGANISM(S): Saccharomyces cerevisiae 
2013-07-03 | E-GEOD-47661 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of mRNA isoform half-lives: identification of stabilizing and destabilizing elements in yeast
We measured half-lives of 21,248 mRNA 3’ isoforms in yeast by rapidly depleting RNA polymerase II from the nucleus and performing direct RNA sequencing throughout the decay process. Interestingly, the half-lives of mRNA isoforms from the same gene, including nearly identical isoforms, often vary wid...
ORGANISM(S): Saccharomyces cerevisiae 
2014-04-02 | E-GEOD-52286 | biostudies-arrayexpress
GenomicsMapping Novel Chromatin Regions Using Sono-Seq
We found that sonication of crosslinked chromatin produces breaks at non-random sites in DNA and can be used to map sites of high chromatin accessibility when combined with high-throughput tag sequencing using the GA II platform from Illumina. This technique, which we named Sono-Seq, can be a simple...
ORGANISM(S): Homo sapiens 
2008-12-18 | E-GEOD-14022 | biostudies-arrayexpress
TranscriptomicsSystematic evaluation of variability in simulated ChIP-chip experiments
The most widely-used method for detecting and measuring genome-wide protein-DNA interactions is chromatin immunoprecipitation on tiling microarrays, commonly known as ChIP-chip. Many tiling array platforms, amplification methods, and analysis algorithms exist for ChIP-chip, but a rigorous assessment...
ORGANISM(S): Homo sapiens 
2008-01-31 | E-GEOD-9842 | biostudies-arrayexpress
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