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To look for Pab1 interacting proteins, GFP-Pab1 was purified from S. pombe cell under nitrogen depletion (cultureed in EMM2-N for 1hour) and subjected to LC-MS/MS analysis.
ORGANISM(S): Schizosaccharomyces pombe 
2024-12-02 | PXD047579 | Pride
We performed mass-spectrometry analysis of proteins co-purifying with Whi5, a predicted repressor of the MBF transcriptional complex. Whi5 was tagged with GFP at its C-terminal end and expressed from its endogenous promoter at its chromosomal locus. Whi5-GFP was immunoprecipitated from cells growing...
ORGANISM(S): Schizosaccharomyces pombe 927 
2025-12-20 | PXD057886 | Pride
Cell competition promotes the elimination of weaker cells from a growing population. Here we investigate how cells of Drosophila wing imaginal discs distinguish “winners” from “losers” during cell competition. Using genomic and functional assays we have identified Maxwell`s Demon (Mwd), a cell membr...
ORGANISM(S): Drosophila melanogaster 
Transcription is a major obstacle for replication fork progression and a cause of genome instability. Such instability increases in mutants with a suboptimal assembly of the nascent messenger ribonucleo-protein particle (mRNP), as THO/TREX and some heterogeneous nuclear ribonucleoproteins (hnRNPs) m...
ORGANISM(S): Saccharomyces cerevisiae 
Variations in the susceptibility to SARS-CoV-2 infection and risk of severe disease are yet not well understood. The information on the human pathways and active members of the microbiome that can affect SARS-CoV-2 susceptibility and disease progression in the saliva is very scarce. Here, we studied...
ORGANISM(S): Homo sapiens (Human) 
2024-05-31 | PXD032333 | Pride
We use high-density tiling DNA microarray technology to obtain the specific transcriptome-wide response induced by 5-Fluorouracil (5FU) in the eukaryotic model Schizosaccharomyces pombe. Schizosaccharomyces pombe cultures with 5FU added to a final concentration of 500 M-NM-
ORGANISM(S): Schizosaccharomyces pombe 
The ING familiy of tumor suppressor proteins control several cellular functions relevant to anti-tumor protection, like cell-cycle control, apoptosis, senescence or migration. ING proteins are functionally linked to the p53 pathway, and they participate in transcriptional control via the recognisit...
ORGANISM(S): Homo sapiens 
Transcription is a major obstacle for replication fork progression and a cause of genome instability. Such instability increases in mutants with a suboptimal assembly of the nascent messenger ribonucleo-protein particle (mRNP), as THO/TREX and some heterogeneous nuclear ribonucleoproteins (hnRNPs) m...
ORGANISM(S): Saccharomyces cerevisiae 
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