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ProteomicsAnalysis of the peptidome of the moss Physcomitrella patens
We performed the analysis of native peptides pool of gametophores, protonema and protoplast cells of the moss. To reduce endogenous proteolytic activity we used an acid extraction buffer with a mixture of plant protease inhibitors. All the actions were performed on ice. After the proper sample prepa...
ORGANISM(S): Physcomitrella patens 
2016-05-18 | PXD000227 | Pride
Plant peptidome Moss Endogenous peptide Physcomitrella patens 
GenomicsThuidium delicatulum isolate:A kind of moss
Thuidium delicatulum isolate:A kind of moss Genome sequencing
PRJNA577519 | ENA
TranscriptomicsGenome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens
Bryophytes are the most basal of the extant land plants. A major feature of these plants is the biphasic alteration of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. To analyse the differences in the transcriptome of the early gametophyte (protonema) and ear...
ORGANISM(S): Physcomitrella patens 
2013-07-29 | E-GEOD-32928 | biostudies-arrayexpress
ProteomicsMoss endogenous peptides, LC-MS/MS
Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often cons...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-08-08 | PXD007922 | Pride
Moss physcomitrella patens tissues 
ProteomicsMoss Physcomitrella paten endogenous peptides, LC-MS/MS, part 2
Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often cons...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-08-08 | PXD007923 | Pride
Moss tissues 
ProteomicsThe moss Physcomitrella patens secretome peptides under chitosan treatment
Taking into account that immune signaling peptides are often species-specific and cannot be predicted based on similarity search, we performed mass spectrometry analysis of the P. patens secretomes. Since the moss P.patens is unable to perceive the known bacterial elicitors such as flagellin or elf1...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2021-03-18 | PXD021721 | Pride
Moss Peptidome Lc-ms/ms 
ProteomicsMoss secreted peptidome - Salicylic acid influences the protease activity and posttranslation modifications of the secreted peptides in the moss Physcomitrella patens
Plant secretome comprise dozens of secreted proteins and peptide hormones. However, little is known about peptide composition of plant secretome as well as proteases responsible for peptide pools generation. While the majority of studies focuses on the target detection and characterization of speci...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-01-02 | PXD010964 | Pride
Physcomitreella patens Sa Lc-ms/ms 
ProteomicsExpression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
Production of biopharmaceuticals relies on the expression of mammalian cDNAs in host organisms. Here we show that the expression of a human cDNA in the moss Physcomitrium patens generates the expected full-length and four additional transcripts due to unexpected splicing. This mRNA splicing results ...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2022-01-10 | PXD026651 | Pride
Aberrant mrna-splicing Heterologous expression systems Physcomitrium patens Blood coagulation factor ix 
ProteomicsTranscriptomic and proteomic analysis of alternative splicing in Physcomitrella patens moss
Alternative splicing (AS) has a significant potential to impact on the eukaryotic cell transcriptome and proteome. However, the extent of this influence as well as mechanisms, providing the tissue-specific pattern of AS are poorly studied. Here, we analyzed the AS of two life forms, protonema and g...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2018-01-15 | PXD005223 | Pride
Alternative splicing Physcomitrella patens moss Long non-coding rnas Proteome 
TranscriptomicsExpression profiling of the moss F. hygrometrica
We experimentally generated a genome-wide gene expression data. To this end, we first collected samples of three important haploid developmental stages, specifically germinating spores (gametophyte_1), protonemata (gametophyte_2) and young gametophores (gametophyte_3) (four biological replicates eac...
ORGANISM(S): Funaria hygrometrica 
2013-07-17 | E-MTAB-1664 | biostudies-arrayexpress
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