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TranscriptomicsGenome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens
Bryophytes are the most basal of the extant land plants. A major feature of these plants is the biphasic alteration of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. To analyse the differences in the transcriptome of the early gametophyte (protonema) and ear...
ORGANISM(S): Physcomitrella patens 
2013-07-29 | E-GEOD-32928 | biostudies-arrayexpress
TranscriptomicsSmall RNAs from dcl3 and rdr6 mutants of Physcomitrella
Endogenous 24nt short interfering RNAs (siRNAs) derived mostly from intergenic and repetitive genomic regions constitute a major class of endogenous small RNAs in Arabidopsis thaliana. Accumulation of A. thaliana 24nt siRNAs requires the Dicer family member DCL3, and clear homologs of DCL3 exist in...
ORGANISM(S): Physcomitrella patens 
2008-12-19 | E-GEOD-12468 | biostudies-arrayexpress
ProteomicsAnalysis of the peptidome of the moss Physcomitrella patens
We performed the analysis of native peptides pool of gametophores, protonema and protoplast cells of the moss. To reduce endogenous proteolytic activity we used an acid extraction buffer with a mixture of plant protease inhibitors. All the actions were performed on ice. After the proper sample prepa...
ORGANISM(S): Physcomitrella patens 
2016-05-18 | PXD000227 | Pride
Plant peptidome Moss Endogenous peptide Physcomitrella patens 
TranscriptomicsGenome-wide transcriptome analysis of gametophyte development in Physcomitrella patens
Gene expression was analyzed with digital gene expression tag profiling (DGEP) with samples taken from P. patens protonema at 3, 14 and 24 days, and also from or leafy shoot tissues l at 30 days after protoplastes isolated, and from 14-day-old caulonemal and chloronemal tissues. Totally, 4333 genes ...
ORGANISM(S): Physcomitrella patens 
2011-12-20 | E-GEOD-33279 | biostudies-arrayexpress
TranscriptomicsDEK1 deletion effect on Physcomitrella patens transcriptome
The main phenotype observed in DEK1 deletion (deltadek1) in P. patens is an absence of gametophore (leafy shoot) development. But, bud initiation the first step in gametophore development occurs. This experiments aims to compare transcriptome of wild-type and deltadek1 strains before budding onset,...
ORGANISM(S): Physcomitrella patens 
2014-10-22 | E-MTAB-2588 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of moss Physcomitrella patens subjected to water stress by means of mannitol, NaCl, abscisic acid and drought
Experiment to identify genes for responding to water stress in the moss Physcomitrella patens using various available water stresses such as mannitol, NaCl, and drought. Treatments were carried out on duplicate tissue samples. RNA isolated independently from each tissue sample and monitored for stre...
ORGANISM(S): Physcomitrella patens 
2008-02-23 | E-TABM-225 | biostudies-arrayexpress
TranscriptomicsAn ABA response regulator unique to basal land plants required for the acquisition of desiccation tolerance
Purpose: The plant hormone abscisic acid (ABA) coordinates responses leading to desiccation tolerance in all land plants. RNA-seq was employed to obtain differential expression data in response to ABA, dehydration and mannitol treatments in wild-type tissue. This was compared against the targeted ge...
ORGANISM(S): Physcomitrella patens 
2016-07-25 | E-GEOD-72583 | biostudies-arrayexpress
TranscriptomicsThe DNA damage response transcriptome of the moss Physcomitrella patens
The moss Physcomitrella patens is remarkable for the ease with which mutant alleles of any gene can be generated by highly efficient homologous recombination-mediated gene targeting. Targeted transgene integration is believed to be mediated through the capture of transforming DNA by the homologous r...
ORGANISM(S): Physcomitrella patens 
2010-11-10 | E-GEOD-25237 | biostudies-arrayexpress
ProteomicsThylakoid protein phosphorylation in Physcomitrella patens
Physcomitrella patens is a model plant representing the group of mosses, which diverged from vascular plant ancestors early after land colonization. To investigate the unique characteristic of the composition and post-translational regulation of Physcomitrella patens photosynthetic apparatus, we hav...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2019-05-08 | PXD012501 | Pride
Physcomitrella patens  moss  thylakoids  photosynthesis  photosystem ii  stn8 kinase  protein phosphorylation  n-terminal acetylation  nlc-msms 
ProteomicsPhyscomitrella patens protonema endogenous peptides
Peptides are small molecules that play important roles in numerous biological processes. We used mass spectrometry to comprehensively analyze the endogenous peptide pools generated from functionally active proteins inside the cell from the model plant Physcomitrella patens. The secretomeand cellular...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2018-12-14 | PXD009532 | Pride
Phytohormones Endogenous peptides Lc-ms/ms Physcomitrella patens 
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