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MetabolomicsExamining the effects of cigarette smoke on mouse lens through a multi OMIC approach
Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for...
2024-03-24 | MTBLS207 | MetaboLights
MetabolomicsThe role of FYCO1-dependent autophagy in lens fiber cell differentiation.

FYCO1 (FYVE and coiled-coil domain containing 1) is an adaptor protein, expressed ubiquitously and required for microtubule-dependent, plus-end-directed transport of macroautophagic/autophagic vesicles. We have previously shown that loss-of-function mutations in FYCO1 cause cataracts wit...

2024-03-24 | MTBLS206 | MetaboLights
TranscriptomicsChromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue]
Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific alphaA-crystallin promoter in postmitotic lens fiber cells. Morpholog...
ORGANISM(S): Mus musculus 
2011-01-01 | E-GEOD-22322 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fiber cell maturation in the lens
The mammalian lens consists of an aged core of quiescent cells enveloped by a layers of mature fully elongated and younger, continuously elongating and transcriptionaly active cells. In the present study, we compared global gene expression profiles in elongating and mature, non-elongating fiber cell...
ORGANISM(S): Mus musculus 
2008-06-22 | E-GEOD-2083 | biostudies-arrayexpress
ProteomicsTranscriptome, proteome, and metabolome of lens fiber cells from mice exposed to cigarette smoke
We adopted an omics (transcriptome, proteome, and metabolome) approach to characterize the lens fiber cells extracted from control (CT) and cigarette smoke (CS) exposed mouse (C57BL/6) eyes. The eight pregnant female mice (gestation days 19-20) were placed in a whole-body exposure smoking chamber an...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD020421 | Pride
Mass-spectrometry Mouse lens fiber cells Tmt Proteome Cigarette smoke 
ProteomicsProteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers
Epithelial cells and differentiated fiber cells represent distinct compartments in the ocular lens. While previous studies have revealed proteins that are preferentially expressed in epithelial vs. fiber cells, a comprehensive proteomics library comparing the molecular composition of epithelial vs. ...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-05 | PXD009639 | Pride
Transcriptome Mouse lens Mass spectrometry Differentiation Transcription factors Proteome Rna-seq 
TranscriptomicsEctopic activation of Wnt/beta-catenin signaling in lens fiber cells results in cataract formation.
Transgenic mouse line called alphaA-CLEF in which transactivation domain of beta-catenin is fused to the amino terminus of DNA-binding protein LEF and the expression of transgenic protein is controlled by alphaA-crystallin promoter (-342/+49) was used to mimic the activation of canonical Wnt/beta-c...
ORGANISM(S): Mus musculus 
2014-02-11 | E-MTAB-1691 | biostudies-arrayexpress
ProteomicsTranscriptome, proteome, and metabolome of lens fiber cells from mice exposed to cigarette smoke
We adopted an omics (transcriptome, proteome, and metabolome) approach to characterize the lens fiber cells extracted from control (CT) and cigarette smoke (CS) exposed mouse (C57BL/6) eyes. The eight pregnant female mice (gestation days 19-20) were placed in a whole-body exposure smoking chamber an...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD017414 | Pride
Mouse Metabolome Mass spectromtery Proteome Transcriptome 
TranscriptomicsChromatin Remodeling Enzyme Smarca5/Snf2h Regulates Cell Cycle Exit, Differentiation of the Lens Epithelium, and Denucleation of Lens Fiber Cells
Genome-wide approach to identify the cell-autonomous role of Snf2h in lens fiber cell terminal differentiation. Differential gene expression was analyzed in Snf2h lens-conditional knockout and wildtype newborn mouse eyeballs, with subsequent comparison of this data with the Brg1 lens-conditional kno...
ORGANISM(S): Mus musculus 
2012-10-18 | E-GEOD-41608 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of Tdrd7 knockdown in lens epithelial-derived cell line 21EM15
Analysis of Tdrd7 deficiency in mouse lens epithelial-derived cell line at gene expression level. The hypothesis tested was that Tdrd7 is involved in post-transcriptional control of gene expression in the lens. Results provide evidence for differential regulation of genes involved in lens homeostasi...
ORGANISM(S): Mus musculus 
2012-02-21 | E-GEOD-25774 | biostudies-arrayexpress
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