We studied the phosphorylation of Arabidopsis LAZY4 proteins during gravistimulation, and searched for its interaction proteins using Immunoprecipitation-Mass Spectrometry (IP-MS). For the identified kinases MPK3 and MKK5, we carried out in vitro kinase assays using recombinant LAZY4 as the substrat...
In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4 and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. While some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identif...