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ProteomicsMacro-porous Reversed Phase Separation of Proteins Combined with Reversed Phase Separation of Phosphopeptides and Tandem Mass Spectrometry for Profiling the Phosphoproteome of MDA-MB-231 cells
A new method of combining macro-porous reversed phase (mRP) protein fractionation with reversed phase liquid chromatography (RPLC) peptide separation tandem mass spectrometry (MS/MS) is reported for profiling the phosphoproteome of a complex sample. In this method, an mRP-C18 column was used to frac...
ORGANISM(S): Homo sapiens (Human) 
2014-07-24 | PXD000948 | Pride
Phosphoproteome Mrp-rplc Scx-rplc Mda-mb-231 Mass spectrometry 
ProteomicsOnline non-contiguous fractionating and concatenating device coupled to two dimensional reverse phase/reverse phase liquid chromatography system for efficient and comprehensive proteomic analyses
The goal of the project is development of an online two dimensional reverse phase/reverse phase liquid chromatography (2D-RP/RPLC) separation platform, employing an online non-contiguous fractionating and concatenating device (NCFC fractionator) in order to provide highly improve separation orthogon...
ORGANISM(S): Homo sapiens (Human) 
2016-12-22 | PXD004710 | Pride
2d-rp/rplc Lc-ms/ms 
ProteomicsNovel Diagnostic Biomarkers for High-Grade Serous Ovarian Cancer Uncovered by Data-Independent Acquisition Mass Spectrometry
High-grade serous ovarian cancer (HGSOC) represents the major histological type of ovarian cancer (OC), and lack of effective screening tools and early detection methods significantly contributes to the poor prognosis of HGSOC. Currently, there is no reliable diagnostic biomarkers for HGSOC. In this...
ORGANISM(S): Homo sapiens (Human) 
2022-08-11 | PXD033169 | Pride
Proteomics Mass spectrometry Data-independent acquisition Serum biomarker High-grade serous ovarian cancer 
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