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ProteomicsMacro-porous Reversed Phase Separation of Proteins Combined with Reversed Phase Separation of Phosphopeptides and Tandem Mass Spectrometry for Profiling the Phosphoproteome of MDA-MB-231 cells
A new method of combining macro-porous reversed phase (mRP) protein fractionation with reversed phase liquid chromatography (RPLC) peptide separation tandem mass spectrometry (MS/MS) is reported for profiling the phosphoproteome of a complex sample. In this method, an mRP-C18 column was used to frac...
ORGANISM(S): Homo sapiens (Human) 
2014-07-24 | PXD000948 | Pride
Phosphoproteome Mrp-rplc Scx-rplc Mda-mb-231 Mass spectrometry 
ProteomicsReverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis
Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...
ORGANISM(S): Homo sapiens (Human) 
2022-06-16 | PXD023817 | Pride
Scx-tip Mass spectrometry Fractionation Chemical cross-linking 
ProteomicsStrong cation exchange-reversed phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry platform with high peak capacity for deep bottom-up proteomics
Two-dimensional (2D) liquid chromatography (LC)-tandem mass spectrometry (MS/MS) are typically employed for deep bottom-up proteomics, and the state-of-the-art 2D-LC-MS/MS has approached over 8,000 protein identifications (IDs) from mammalian cell lines or tissues in 1-3 days of mass spectrometer ti...
ORGANISM(S): Mus musculus (Mouse) 
2018-02-27 | PXD008432 | Pride
Strong cation exchange  reversed-phase liquid chromatography  capillary zone electrophoresis  tandem mass spectrometry  bottom-up proteomics 
UnknownCapillary isoelectric focusing-tandem mass spectrometry and reversed-phase liquid chromatography-tandem mass spectrometry for quantitative proteomic analysis of differentiating PC12 cells by eight-plex isobaric tags for relative and absolute quantification.
Not available
S-EPMC3759156 | biostudies-literature
UnknownStrong cation exchange-reversed phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry platform with high peak capacity for deep bottom-up proteomics.
Not available
S-EPMC5831384 | biostudies-literature
ProteomicsProteomic profiling of Emiliania huxleyi
Proteomic profiling of Emiliania huxleyi was performed using a 3D-LC method.
ORGANISM(S): Emiliania huxleyi CCMP371 
2020-10-13 | PXD018511 | Pride
Proteomic profiling Emiliania huxleyi Q exactive Scx Rplc 
ProteomicsProteomic Analysis of Human Liver Reference Material
Recent advances in mass spectrometric (MS) instruments resulting in high mass resolution and high duty cycles have allowed of very deep coverage of proteomes. However, even with state of the art, high duty cycle instruments, the number of proteins identified with a conventional single liquid chromat...
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD009021 | Pride
Human Liver 
ProteomicsSCX_MS raw files and RP_MS raw files
A novel one-dimensional on-line pH gradient-eluted strong cation exchange (SCX)-nano-ESI-MS/MS method was developed for protein identification and tested with mixture of six standard proteins, total lysate of HuH7 and N2a cells, as well as membrane fraction of N2a cells. This method utilized an on-l...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-01-28 | PXD003438 | Pride
Strong cation exchange Ph gradient 
UnknownFully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses.
Not available
S-EPMC3412908 | biostudies-literature
ProteomicsUnderstanding bovine germinal vesicle (GV) oocyte and cumulus cell communication... A systems biology approach
Triplicate samples of oocyte and cumulus cells were isolated from follicles of 2-8 mm, containing oocytes with several layers of cumulus cells were collected from ovaries. Proteins were extracted using DDF and digested using trypsin. The peptides were desalted, separated using in-line SCX-RPLC and a...
ORGANISM(S): Bos taurus (Bovine) 
2011-04-15 | PRD000082 | Pride
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