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ProteomicsExtending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080845 | MassIVE
TranscriptomicsExpression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice.
Purpose: Our lab has previously shown that Scleraxis (Scx) is require for proper valve development in vivo. In order to fully explore gene networks regulated by Scx during the vital stages of valve remodeling , high throughput RNA-squencing was performed. Results:There were a total of 18,810 genes w...
ORGANISM(S): Mus musculus 
2014-05-14 | E-GEOD-57423 | biostudies-arrayexpress
ProteomicsReverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis
Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...
ORGANISM(S): Homo sapiens (Human) 
2022-06-16 | PXD023817 | Pride
Scx-tip Mass spectrometry Fractionation Chemical cross-linking 
GenomicsRNA-seq analysis of equine tenocytes exposed to siRNA targeting scleraxis (Scx) to identify novel targets of Scx-mediated mechanotransduction
RNA-seq analysis of equine tenocytes exposed to siRNA targeting scleraxis (Scx) to identify novel targets of Scx-mediated mechanotransduction
PRJNA433994 | ENA
GenomicsMultiple tissue sequencing of WT and Scx-ko mice
Multiple tissue sequencing of WT and Scx-ko mice
PRJNA578109 | ENA
TranscriptomicsExpression data from mouse limb tendon cells during development.
We have undertaken a screen of mouse limb tendon cells in order to identify molecular pathways involved in tendon development. Mouse limb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out ...
ORGANISM(S): Mus musculus 
2014-01-18 | E-GEOD-54207 | biostudies-arrayexpress
GenomicsGenome-wide identification of Scx transcriptional target genes in tendon development II
Genome-wide identification of Scx transcriptional target genes in tendon development II
PRJNA725501 | ENA
TranscriptomicsExpression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice.
Purpose: Our lab has previously shown that Scleraxis (Scx) is require for proper valve development in vivo. In order to fully explore gene networks regulated by Scx during the vital stages of valve remodeling , high throughput RNA-squencing was performed. Results:There were a total of 18,810 genes w...
ORGANISM(S): Mus musculus 
2014-05-14 | GSE57423 | GEO
TranscriptomicsGenome-wide identification of Scx transcriptional target genes in tendon development II
Despite their important roles in the musculoskeletal system, tendon and ligaments are much less studied comparing to bone, cartilage and muscle. The lack of knowledge in tendon biology severely hinders the understanding of the etiologies of tendon related diseases and development of efficient clinic...
ORGANISM(S): Mus musculus 
2021-06-28 | GSE173428 | GEO
ProteomicsExtending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo sapiens (Human) 
2014-09-30 | PXD000705 | Pride
2d lc-ms/ms Proteomics 
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