Sort   by:  
 Page size 
ProteomicsReverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis
Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...
ORGANISM(S): Homo sapiens (Human) 
2022-06-16 | PXD023817 | Pride
Scx-tip Mass spectrometry Fractionation Chemical cross-linking 
ProteomicsOne-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Large-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation site...
ORGANISM(S): Homo Sapiens (human) 
2024-08-13 | PXD048917 | JPOST Repository
UnknownOne-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography.
Not available
S-EPMC11382313 | biostudies-literature
UnknownRapid and Efficient Enrichment of Snake Venoms from Human Plasma Using a Strong Cation Exchange Tip Column to Improve Snakebite Diagnosis.
Not available
S-EPMC7917991 | biostudies-literature
ProteomicsAn Optimized Miniaturized Filter Aided Sample Preparation Method for Sensitive Cross-linking Mass Spectrometry Analysis of Microscale Samples
Cross-linking mass spectrometry (XL-MS) is a powerful tool for elucidating protein structures and protein-protein interactions (PPIs) at the global scale. However, sensitive XL-MS analysis of mass-limited samples remains challenging, due to serious sample loss during sample preparation of the low-ab...
ORGANISM(S): Homo sapiens (Human) 
2025-01-03 | PXD051322 | Pride
Cross-linking mass spectrometry Microscale samples 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
ProteomicsPurification of Biotinylated Peptides using SDB-SCX StageTip
We tried purification of biotinylated peptides using SDB-SCX StageTip to reduce interference ions. We compared this method with the original and optimized workflows. We assessed reproducibility of each enrichment process by three technical replicates. Original workflow with SDB-SCX: A 15-µL slurry o...
ORGANISM(S): Mus Musculus (mouse) 
2022-08-25 | PXD035229 | JPOST Repository
ProteomicsProtease specificity profiling in a pipette tip using “charge-synchronized” proteome-derived peptide libraries
About 2% of the genome of human and other organisms codes for proteases. An important step toward deciphering the biological function of a protease and designing inhibitors is the profiling of protease specificity. In this work we present a novel, label-free, proteomics-based protease specificity pr...
ORGANISM(S): Escherichia coli 
2018-04-26 | PXD007556 | Pride
Charged-synchronized peptide library Protease specificity Chafratip Label-free 
UnknownTwo-Dimensional Fractionation Method for Proteome-Wide Cross-Linking Mass Spectrometry Analysis.
Not available
S-EPMC9056026 | biostudies-literature
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
Membrane proteins are crucial targets for cancer biomarker discovery and drug development. However, in addition to the inherent challenges of hydrophobicity and low abundance, complete membrane proteome coverage of clinical specimen is usually hindered by the requirement of large amount of starting ...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2015-11-16 | PXD001548 | Pride
High-ph reverse phase stagetip Membrane proteins Lung cancer 
Sort   by:  
 Page size