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ORGANISM(S): Homo sapiens 
Optimization of intact protein TMT labeling and multidimensional high-pH/low-pH RP/RPLC-MS/MS. Application of this platform for quantification of proteoform-level abundance changes as a result of staursporine treatment in HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2026-08-13 | PXD078120 | Pride
The studied compound SN12 significantly inhibited biofilm formation in Pseudomonas aeruginosa, but its regulatory mechanism is unknown. To determine the mechanism of action, TMT tagging was used to analyse the proteomics of P. aeruginosa PAO1. Three groups of blank controls treated with PBS were com...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2025-05-06 | PXD047035 | Pride
A new method of combining macro-porous reversed phase (mRP) protein fractionation with reversed phase liquid chromatography (RPLC) peptide separation tandem mass spectrometry (MS/MS) is reported for profiling the phosphoproteome of a complex sample. In this method, an mRP-C18 column was used to frac...
ORGANISM(S): Homo sapiens (Human) 
2014-07-24 | PXD000948 | Pride
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. Howe...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD009991 | Pride
The goal of the project is development of an online two dimensional reverse phase/reverse phase liquid chromatography (2D-RP/RPLC) separation platform, employing an online non-contiguous fractionating and concatenating device (NCFC fractionator) in order to provide highly improve separation orthogon...
ORGANISM(S): Homo sapiens (Human) 
2016-12-22 | PXD004710 | Pride
Development of low-flow liquid-chromatography setup by combining hydrophylic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC) and coupled with a high resolution mass spectrometer.
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2021-03-29 | PXD022330 | Pride
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