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ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, rice
The associated files are mass spec data from individual fractions of mixed-bed ion exchange or size exclusion fractionations of native extract made from rice leaves (Oryza sativa, Kitaake cultivar).
ORGANISM(S): Oryza sativa (Rice) 
2019-10-28 | PXD013213 | Pride
Ion exchange Size exclusion Oryza sativa kitaake Rice 
ProteomicsProteomics of Pseudomonas aeruginosa PAO1 and PA14
Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) a...
ORGANISM(S): Pseudomonas aeruginosa PA14 Pseudomonas aeruginosa PAO1 
2014-05-08 | PXD000684 | Pride
Synthetic cystic fibrosis medium Pseudomonas aeruginosa Msblender 
ProteomicsRotifer proteome from SEC fractionated Brachionus rotundiformis
Brachionus rotundiformis were obtained by filtration of a non-axenic culture to remove feeder algae. Rotifers were flash frozen, ground in liquid nitrogen, and lysed by resuspension in buffer containing 0.2% NP40 with dounce homogenization. Native protein lysate was fractionated on an HPLC SEC and...
ORGANISM(S): Brachionus Rotundiformis (ncbitaxon:96890) 
2023-04-11 | MSV000091679 | MassIVE
ProteomicsEPIC: Software Toolkit for Elution Profile-Based Inference of Protein Complexes
Heteromeric protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to preci...
ORGANISM(S): Caenorhabditis elegans 
2019-07-16 | PXD011182 | Pride
Cf/ms  epic  biochemical fractionation  worm interaction network  mass spectrometry  proteomics  protein complexes 
ProteomicsProteomics of SEC and IEX fractionated Mouse Embryonic Stem Cells
Mouse embryonic stem cells were lysed in Pierce IP Lysis buffer and 2 mg of the clarified extract was fractionated on an HPLC SEC column and 2 mg was fractionated on an HPLC IEX mixed bed column. All fractions were reduced, alkylated, and trypsin digested for mass spectrometry as in Mallam et al. 2...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-05-01 | MSV000091831 | MassIVE
ProteomicsProtein Identification in Tetrahymena thermophila cilia IEX fractions used for XL-MS
Tetrahymena thermophila SB715 were deciliated by pH shock substituting HEPES for Tris in all subsequent buffers. Ciliry proteins were solubilized with buffer containing 1%NP40, and fractionated by HPLC on a mixed-bed IEX column. DSSO was added to 0.5 mM to all collected fractions. Crosslinking wa...
ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911) 
2023-04-13 | MSV000091710 | MassIVE
ProteomicsPanorama of ancient metazoan macromolecular complexes: Saccharomyces cerevisiae
By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.me...
ORGANISM(S): Saccharomyces cerevisiae W303 Saccharomyces cerevisiae (Baker's yeast) 
2015-06-29 | PXD002327 | Pride
Lc-msms Conservation Metazoan Biofractionation Across species 
ProteomicsPanorama of ancient metazoan macromolecular complexes: Human, Cell paper 2012: MOCK
By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.me...
ORGANISM(S): Homo sapiens (Human) 
2016-05-18 | PXD002328 | Pride
Lc-msms Conservation Metazoan Biofractionation Across species 
ProteomicsPanorama of ancient metazoan macromolecular complexes: Xenopus laevis
By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.me...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2015-06-22 | PXD002326 | Pride
Lc-msms Conservation Metazoan Biofractionation Across species 
ProteomicsPanorama of ancient metazoan macromolecular complexes: Sea urchin
By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.me...
ORGANISM(S): Strongylocentrotus purpuratus (Purple sea urchin) 
2015-06-19 | PXD002325 | Pride
Lc-msms Conservation Metazoan Biofractionation Across species 
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