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ProteomicsTMTpro 16-plex analysis of siRAD23B and siControl in replicatively and ETP-treated senescent cells
Replicatively-senescent and ETP-treated senescent WI-38 cells were transfected with siRNAs (Stealth Select RNAi RAD23B siRNA #1 and siGENOME non-targeting control siRNA #2) using Lipofectamine RNAiMAX in four biological replicates. At 72 h after transfection, the cells were washed twice with cold-PB...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD028626 | JPOST Repository
ProteomicsTMT8plex_bRP_WI38_Ctr_Aging_Btz_Etp
WI-38 cells were washed three times with PBS, and cell lysis, trypsin/Lys-C digestion, and peptide purification were performed using the EasyPep Mini MS sample prep kit (Thermo Fisher Scientific). Peptides (25 μg) from each sample were labeled with 0.2 mg of TMT 10-plex reagents (Thermo Fisher Scien...
ORGANISM(S): Homo Sapiens (human) 
2022-10-21 | PXD029271 | JPOST Repository
ProteomicsInteractome analysis for Gore-tex/Osiris23 and identification of ubiquitination sites in Gore-tex/Osiris23
To identify the proteins interacting with Gore-tex/Osiris23, affinity purification mass spectrometry (AP-MS) was performed using S2 cells transfected with HA or Flag-tagged Gore-tex/Osiris23 expression vectors. PTM analysis was also performed to determine the ubiquitination sites of Gore-tex/Osiris2...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2026-03-12 | PXD053779 | JPOST Repository
ProteomicsLFQ_TurboID-PSMA6_ETP_Tamavidin2-REV
The control and ETP-treated WI-38 cells stably expressing α1-TurboID were seeded and cultured overnight. The cells were treated with 500 μM biotin for 1 h. Preparation of cell lysates, tryptic digestion, and enrichment of biotinylated peptides using Tamavidin 2-REV were performed as described previo...
ORGANISM(S): Homo Sapiens (human) 
2022-10-21 | PXD029272 | JPOST Repository
ProteomicsTurboID-mediated proximal labeling of Drosophila BubR1 and its cleavage variants
V5-TurboID-tagged Drosophila BubR1 and its cleavage variants were transiently expressed in Drosophila S2 cells in two biological replicates. Cells were treated with 500 µM biotin for 30 min for protein biotinylation. Biotinylated protein-containing lysate was subjected to FG-NeutrAvidin beads purifi...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2023-05-11 | PXD033797 | JPOST Repository
ProteomicsTMT10plex_cTEC_mTEC_beta5tKO-cTEC
TEC cells (K5D1 cTECs in quadruplicate, K5D1 mTECs in triplicate, and K5D1-β5tKO cTECs in triplicate) were lysed in 6 M guanidine-HCl containing 100 mM Tris-HCl, pH 8.0, and 2 mM DTT. The clarified lysates were reduced in 5 mM DTT and alkylated in 27.5 mM iodoacetamide. Proteins were purified by met...
ORGANISM(S): Mus Musculus (mouse) 
2019-10-08 | PXD013132 | JPOST Repository
ProteomicsLFQ_K5D1-cTEC_K5D1-beta5tKO-cTEC
Cortical TECs from K5D1 and K5D1-β5tKO mice were lysed, reduced, alkylated, and subjected to methanol/chloroform precipitation. Proteins were digested with trypsin/Lys-C mix. The digests were desalted using GL-Tip SDB, and the eluates were evaporated in a SpeedVac concentrator and dissolved in 0.1% ...
ORGANISM(S): Mus Musculus (mouse) 
2019-10-08 | PXD013133 | JPOST Repository
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