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ProteomicsBacterial response to glyphosate-based herbicide Roundup stress
This project aimed to investigate the effects of glyphosate-based herbicide Roundup LB Plus on bacteria. For this, ten environmental strains of Salmonella enterica were exposed to the increasing concentrations of Roundup over several passages to obtain Roundup-resistant mutants. Four stable re-seque...
ORGANISM(S): Bacteria Salmonella enterica subsp. enterica serovar Enteritidis str. EC20120200 
2020-09-22 | PXD019463 | Pride
Experimental evolution Resistance Label free quantitative proteomics Whole cell protein extraction Bacterial stress response Salmonella enterica Roundup Glyphosate 
ProteomicsComparative proteomics analysis of microalgae Prototheca associated with severe, mild and non-infectious strains
Three strains, P. zopfii genotype 1 (SAG 2063), P. zopfii genotype 2 (SAG 2021) and P. blaschkeae (SAG 2064) from the culture collection at the Institute of Animal Hygiene and Environmental Health, Freie Universität Berlin, Germany were cultured six times independently at 37°C in Sabouraud dextrose ...
ORGANISM(S): Prototheca zopfii 
2017-01-02 | PXD005305 | Pride
Label free quantitative analysis Prototheca blaschkeae Perseus software Prototheca Bovine masititis Prototheca zopfii Comparative proteomic analysis Ltq orbitrap velos mass spectrometer  maxquant 
ProteomicsLabel-free quantitative proteomic analysis of Brucella abortus and Brucella melitensis
Brucella type strains (B. abortus 544 and B. melitensis 16 M), field-isolates (B. abortus T and B. melitensis C) were independently cultivated six times on Tryptic Soy Broth at 37 °C in the presence of 5% CO2 until mid-logarithmic phase. The cells were harvested by brief centrifugation and the bacte...
ORGANISM(S): Brucella abortus Brucella melitensis 
2020-06-11 | PXD006348 | Pride
Label free quantitative analysis Perseus software Brucella abortus Brucella melitensis Maxquant-andromeda Comparative proteomic analysis Ltq orbitrap velos mass spectrometer Maxquant software 
ProteomicsProteomics analysis of milk from cows with Staphylococcus aureus subclinical mastitis
Liquid chromatography-mass spectrometry (LC/MS) based label free quantitative proteomics analysis was applied for identification of differentially expressed proteins among the whey samples isolated from, 1) milk from cows with no history of mastitis, negative for S. aureus and somatic cell count (SC...
ORGANISM(S): Bos taurus (Bovine) Bos taurus 
2018-01-02 | PXD007516 | Pride
Label free quantitative analysis Bovine mastitis Perseus software Staphylococcus aureus Cow milk proteomics Whey Maxquant-andromeda Ltq orbitrap velos mass spectrometer Maxquant software 
ProteomicsPrototheca zopfii genotype proteomics analysis
The present study was initiated to evaluate the quantitative proteomic profiling of Protothecazopfiigenotypes. The cells (P.zopfii genotype 1 –noninfectious and genotype 2 -infectious) were cultured in triplicates until the mid-logarithmic growth phase; the proteins were extracted after sonication o...
ORGANISM(S): Chlorella-like algae Prototheca zopfii 
2013-07-22 | PXD000126 | Pride
Bovine mastitis Maldi tof ms Prototheca Infectious algae 2d-dige 
ProteomicsLabel-free quantitative proteomic analysis of Mycobacterium avium hominissuis
The objective of this study is to investigate the protein expression level difference between Mycobacterium avium hominissuis wild type strain MAH-104 , its mutant 21m (gene lysX (MAV_3128) mutated - Lysyl tRNA synthetase) and the complemented strain 21c (MAV_3128comp) using liquid chromatography-...
ORGANISM(S): Mycobacterium avium complex Mycobacterium avium subsp. hominissuis 
2018-02-26 | PXD006470 | Pride
Label free quantitative analysis Proteomic analysis Mutant Perseus software Mycobacterium avium hominissuis Maxquant-andromeda Ltq orbitrap velos mass spectrometer Maxquant software 
ProteomicsIdentification of immunodominant antigen among Brucella species (B. abortus and B. melitensis) using hyper immune serum from naturally infected hosts
In the present study, the whole cell protein extracts of B. abortus and B. melitensis were separated using SDS-PAGE and western blotting was carried out with the antiserum from naturally infected host animals (cow, buffalo, goat and sheep). The proteins bands that matched with western blot signals w...
ORGANISM(S): Brucella abortus Brucella melitensis Brucella 
2014-12-01 | PXD001270 | Pride
Hyper immune serum Immunodominat proteins Brucella Maldi tof ms Western blotting 
ProteomicsComparative proteomics analysis of Polynucleobacter asymbioticus
Comparative proteomics analysis to evaluate the influence of varying culture conditions on a planktonic freshwater dwelling bacterium, Polynucleobacter asymbioticus that belongs to the class Gammaproteobacteria. In the present study, a type strain Polynucleobacter asymbioticus strain QLW-P1DMWA-1T (...
ORGANISM(S): Polynucleobacter asymbioticus Polynucleobacter asymbioticus QLW-P1DMWA-1 
2020-09-09 | PXD020103 | Pride
Polynucleobacter asymbioticus  gram-negative  aerobic  freshwater habitats  proteomics  ltq orbitrap velos mass spectrometer  maxquant  perseus software 
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