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ProteomicsCRISPR-Cas9/phosphoproteomics identifies Myosin Light Chain Kinase dependent signaling in kidney epithelial cells
Prior studies have implicated myosin light chain kinase (MLCK) in the regulation of aqua-porin-2 (AQP2) in the renal collecting duct. To discover signaling targets of MLCK, we deleted the Mylk- gene to obtain MLCK-null mpkCCD cells and carried out comprehensive phosphopro-teomics using the SILAC met...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-04 | PXD014985 | Pride
Nuclear membrane Mass spectrometry Vasopressin Aqp2 Endosomal trafficking Sted microscopy 
ProteomicsAnalysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins
The myosin light chain protein family consists of two classes, the regulatory myosin light chains (RLC) and the essential myosin light chains (ELC). Functionally, the MLC proteins are directly involved in sarcomeric activity and force transmission contributing to cardiac contractility. Besides regul...
ORGANISM(S): Homo sapiens (Human) 
2022-07-24 | PXD033908 | Pride
Cardiomyocytes Essential myosin light chains (elc) Phosphorylation Regulatory myosin light chains (rlc) 
ProteomicsIn-depth Characterization of S-Glutathionylation of Ventricular Myosin Light Chain 1 Across Species by Top-Down Proteomics
S-glutathionylation (SSG) is increasingly recognized as a critical signaling mechanism in the heart, yet SSG modifications in cardiac sarcomeric proteins remain understudied. Here we identified SSG of the ventricular isoform of myosin light chain 1 (MLC-1v) in human, swine, and mouse cardiac tissues...
ORGANISM(S): Sus Scrofa (ncbitaxon:9823) Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2024-12-09 | MSV000096622 | MassIVE
TranscriptomicsRNP immunoprecipitation of fission yeast using Rng3p myosin-specific chaperones to investigate if the association of Rng3p with RNAs occurs on ribosomes
Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using the Rng3p myosin-specific chaperones from the fission Schizosaccaromyces pombe. Rng3p associates with RNAs encoding myosins. To investigate if the association of Rng3p with these RNAs occurs on ribosomes, Rng3-purified fractio...
ORGANISM(S): Schizosaccharomyces pombe 
2008-12-23 | E-TABM-564 | biostudies-arrayexpress
TranscriptomicsmiR-487b, miR-3963 and miR-6412 delayed myogenic differentiation in C2C12 mouse myoblast-derived cells.
We newly identified skeletal muscle differentiation-associated miRNAs by comparing miRNA expression profile between C2C12 cell and Wnt4-overexpressing C2C12 cell. miR-487b, miR-3963 and miR-6412 are significantly down-regulated in differentiating C2C12 cells, and transfection of their mimics result...
ORGANISM(S): Mus musculus 
2015-11-24 | E-GEOD-63454 | biostudies-arrayexpress
TranscriptomicsRibonucleoprotein immunoprecipitation of fission yeast to study myosin proteins and other cytoskeletal components
Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and other cytoskeletal components from the fission Schizosaccaromyces pombe
ORGANISM(S): Schizosaccharomyces pombe 
2009-09-01 | E-TABM-656 | biostudies-arrayexpress
UnknownSmooth muscle myosin light chain kinase efficiently phosphorylates serine 15 of cardiac myosin regulatory light chain.
Not available
S-EPMC3242870 | biostudies-literature
ProteomicsAnalysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling
NIMA-related kinase 9 (NEK9) was identified to interact with the essential myosin light chain (ELC). To validate these findings ascorbate peroxidase (APEX) catalyzed proximity labeling (Hung et al. Nat Protoc. 2016) was performed. To do so, human ELC fused to APEX was overexpressed in HEK293 cells. ...
ORGANISM(S): Homo sapiens (Human) 
2022-07-24 | PXD034115 | Pride
Bioid Nek9 Elc 
UnknownMyosin light chain kinase (MLCK) regulates cell migration in a myosin regulatory light chain phosphorylation-independent mechanism.
Not available
S-EPMC4192498 | biostudies-literature
TranscriptomicsMYBPH, a novel transcriptional target of NKX2-1/TTF-1, inhibits ROCK1 and actomyosin assembly, and reduces cell motility and tumor metastasis
Cell migration driven by actomyosin filament assembly is a critical step in tumour invasion and metastasis. Herein, we report identification of myosin binding protein H (MYBPH) as a transcriptional target of NKX2-1 (also known as TTF-1 and TITF1), a lineage-survival oncogene in lung adenocarcinoma. ...
ORGANISM(S): Homo sapiens 
2011-12-22 | E-GEOD-26721 | biostudies-arrayexpress
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