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MetabolomicsN6-methyladenosine modification is not a general trait of positive-sense RNA viral genomes

Despite the nuclear localization of the m6A machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively m6A-modified. However, these findings are mostly based on m6A-seq, an antibody-dependent technique with...

2024-01-25 | MTBLS6978 | MetaboLights
MetabolomicsGut Commensal Bacteria-Derived Methionine is Required for Host Reproduction by Modulating RNA m6A Methylation of the Insulin Receptor_targeted metabolites

Gut commensal bacteria promote host reproduction by modulating metabolism and nutrition, but the molecular mechanisms by which microbes regulate reproduction remain unclear. Here, we show that gut commensal bacteria promote host reproduction by providing amino-acid methionine, which controls the ...

2025-06-22 | MTBLS12470 | MetaboLights
MetabolomicsSystemic Inflammation Induces Cholestasis via Upregulating a Short Variant of FTO to Remove the m6A Methylation in the 3’-UTR of Cyp7b1 mRNAs in liver
The cholestasis frequently occurs in patients with inflammatory processes. However, the mechanisms of inflammation in inducing cholestasis are still unclear. Here, by using lipopolysaccharide (LPS)-induced cholestasis mouse models, we found that most genes encoding enzymes responsible for bile acid ...
2025-11-07 | MTBLS10713 | MetaboLights
Transcriptomicsm6a peak analysis in normal, FTO deficient and five stages of adipogenesis (D-2/0/2/5/10) in Mouse embryo fibroblast 3T3-L1 pre-adipocytes
Total RNA was extracted using TRI® Reagent (Sigma). cDNA was synthesized by RevertAid™ First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturer’s recommendations. PCR reactions were carried out on a DNAEngine® Thermal Cycler (PTC-0200G, Bio-Rad) in 25 μl reacti...
ORGANISM(S): Mus musculus 
2015-03-09 | E-GEOD-53244 | biostudies-arrayexpress
Transcriptomicsm6A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency
N6-methyladenosine (m6A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m6A modification profiles in the mRNA transcriptomes of four cell...
ORGANISM(S): Mus musculus 
2015-03-10 | E-GEOD-52125 | biostudies-arrayexpress
TranscriptomicsIdentification and Characterization of the Mammalian Nuclear RNA N6-Adenosine Methyltransferase Core Complex
N6-methyladenosine (m6A) is the most prevalent internal modification found in mammalian messenger and non-coding RNAs. The discoveries of functionally significant demethylases that reverse this methylation as well as the recently revealed m6A distributions in mammalian transcriptomes strongly indica...
ORGANISM(S): Homo sapiens 
2013-11-29 | E-GEOD-46705 | biostudies-arrayexpress
TranscriptomicsHigh-resolution mapping reveals a conserved, widespread, dynamic meiotically regulated mRNA methylation program [Mm]
N6-methyladenosine (m6A) is a common modification of mRNA, with potential roles in fine-tuning the RNA life cycle, but little is known about the pathways regulating this process and its physiological role. Here, we used mass-spectrometry to identify a dense network of proteins physically interacting...
ORGANISM(S): Mus musculus 
2014-07-18 | E-GEOD-55575 | biostudies-arrayexpress
GenomicsProfiling of mRNA N6-Adenosine Methylation (m6A) in Arabidopsis
m6A profiling in two accessions of Arabidopsis thaliana (Can-0 and Hen-16) using the m6A-targeted antibody coupled with high-throughput sequencing m6A-seq in two accessions of Arabidopsis, two replicates for each sample
ORGANISM(S): Arabidopsis thaliana 
2014-11-06 | E-GEOD-59154 | biostudies-arrayexpress
TranscriptomicsHigh-resolution mapping reveals a conserved, widespread, dynamic meiotically regulated mRNA methylation program [Hs]
N6-methyladenosine (m6A) is a common modification of mRNA, with potential roles in fine-tuning the RNA life cycle, but little is known about the pathways regulating this process and its physiological role. Here, we used mass-spectrometry to identify a dense network of proteins physically interacting...
ORGANISM(S): Homo sapiens 
2014-07-18 | E-GEOD-55572 | biostudies-arrayexpress
TranscriptomicsWidespread Occurrence of m6A in bacterial mRNA
N6-methyladenosine (m6A) is one of the most abundant modifications in eukaryotic RNA. Recent mapping of m6A methylomes in mammals, yeast, and plants as well as characterization of m6A methyltransferases, demethylases, and binding proteins have revealed regulatory functions of this dynamic RNA modifi...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2015-07-08 | E-GEOD-65347 | biostudies-arrayexpress
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