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TranscriptomicsLong-term expandable SOX9+ chondrogenic ectomesenchymal cells from human pluripotent stem cells
Purpose: We have succeeded in the generation and long-term expansion of SOX9-expressing CD271+PDGFRa+CD73+ chondrogenic ectomesenchymal cells from the PAX3/SOX10/FOXD3-expressing MIXL1-CD271hiPDGFRaloCD73- neural crest-like progeny of human pluripotent stem cells in a chemically defined medium suppl...
ORGANISM(S): Homo sapiens 
2015-02-15 | E-GEOD-64752 | biostudies-arrayexpress
ProteomicsTIG-3 quantification of metabolic enzymes set12
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004840 | JPOST Repository
ProteomicsMatsumoto_Verification of MRM assays using in vitro proteome
MS/MS spectra assigned to peptides with more than six amino acids were selected from our database and converted into MRM transitions. Overlapping ions with a difference in mass/charge ratio (m/z) of 400 were preferentially selected
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004902 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set08
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004836 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set09
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004837 | JPOST Repository
ProteomicsEffect of oncogene-induced transformation on metabolic enzyme core set
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004845 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set03
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004831 | JPOST Repository
ProteomicsEffect of oncogenic transformation on metabolism 07
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004844 | JPOST Repository
ProteomicsEffect of oncogenic transformation on metabolism 05
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004850 | JPOST Repository
ProteomicsEffect of oncogenic transformation on metabolism 06
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004851 | JPOST Repository
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