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MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsOnline-2D nanoLC-MS for crude serum proteome profiling: assessing sample preparation impact on proteome composition
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-09 | PXD024893 | Pride
Peptide fractionation Blood Nanolc-ms 1d nanolc-ms Proteome profiling Crude serum High-resolution accurate mass-spectrometry Online-2d nanolc-ms 
ProteomicsBioinertization of nanoLC/MS/MS systems by depleting metal ions from the mobile phases for phosphoproteomics
We have successfully bioinertized a nanoLC/MS/MS system for the highly sensitive analysis of phosphopeptides by depleting metal ions from the mobile phase. We found that not only the direct contact of phosphopeptides with metal materials, but also the indirect contact with HPLC pump through the mobi...
ORGANISM(S): Homo Sapiens (human) 
2023-03-24 | PXD034942 | JPOST Repository
ProteomicsA nanoLC-MS/MS system using a nonporous reverse phase column for ultrasensitive proteome analysis
The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporo...
ORGANISM(S): Homo Sapiens (human) 
2018-09-24 | PXD011168 | JPOST Repository
UnknownBioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics.
Not available
S-EPMC10172917 | biostudies-literature
UnknownUltracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.
Not available
S-EPMC6331754 | biostudies-literature
UnknownIncreasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS.
Not available
S-EPMC3703961 | biostudies-literature
UnknownNanowell-mediated multidimensional separations combining nanoLC with SLIM IM-MS for rapid, high-peak-capacity proteomic analyses.
Not available
S-EPMC6500776 | biostudies-literature
UnknownDeep Single-Shot NanoLC-MS Proteome Profiling with a 1500 Bar UHPLC System, Long Fully Porous Columns, and HRAM MS.
Not available
S-EPMC9552226 | biostudies-literature
UnknownEfficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans.
Not available
S-EPMC4843773 | biostudies-literature
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