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UnknownMultimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS.
Not available
S-EPMC9946438 | biostudies-literature
ProteomicsAutomated coupling of nanodroplet sample preparation with liquid chromatography-mass spectrometry for high throughput single-cell proteomics
Development and evaluation of an autosampler for integrating nanoPOTS with LC-MS. Includes proteomic data from nanoPOTS-based sample preparation, including diluted peptides of Shewanella oneidensis MR-1, single cultured MCF10A cells, and single cells from three Acute Myeloid Leukemia (AML) cell line...
ORGANISM(S): Shewanella Oneidensis Mr-1 (ncbitaxon:211586) Homo Sapiens (ncbitaxon:9606) 
2020-04-05 | MSV000085230 | MassIVE
ProteomicsImproved Single Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry
we report on improved single-cell proteome coverage through the combination of the previously developed Nanodroplet Processing in One Pot for Trace Samples (nanoPOTS) platform with further miniaturization of liquid chromatography (LC) separations and implementation of an ultrasensitive latest-genera...
ORGANISM(S): Homo sapiens (Human) 
2020-02-26 | PXD016921 | Pride
Eclipse Narrow-bore lc Single cell 
ProteomicsSpatially resolved proteome profiling of less than 200 cells from tomato fruit pericarp by integrating laser-capture microdissection with nanodroplet sample preparation
Proteomic data from technical developments of a nanoPOTS-based spatially resolved proteome profiling of <200 cells from tomato fruit pericarp.
ORGANISM(S): Solanum lycopersicum 
2018-08-21 | PXD010806 | Pride
Nanopots Proteomics Spatially resolved 
ProteomicsA comprehensive evaluation of common quantification strategies for LC-MS/MS-based spatial proteomics using nanoPOTS
A deep understanding of the tissue microenvironment can provide significant insight into the function of tissue and organs in health and disease states. Spatially resolved proteomics techniques can aid in studying spatial tissue organization and cellular communication in their native microenvironmen...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-03-22 | MSV000091531 | MassIVE
ProteomicsUltrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
UnknownNanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells.
Not available
S-EPMC5830451 | biostudies-literature
ProteomicsTDP-43-stratified single-cell proteomic profiling of postmortem human spinal motor neurons reveals protein dynamics in amyotrophic lateral sclerosis
Single cell dataset of human motor neurons laser-captured from postmortem ALS and control tissues (1) ALS Pilot dataset (2) TDP43 stratified dataset Summary: Unbiased proteomics has been employed to interrogate central nervous system (CNS) tissues (brain, spinal cord) and fluid matrices (CSF, plas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-06-07 | MSV000092119 | MassIVE
OtherMultimodal Mass Spectrometry Imaging of Rat Brain using IR-MALDESI and nanoPOTS-LC-MS/MS
Multimodal mass spectrometry imaging (MSI) is a critical technique used for deeply investigating biological systems by combining multiple MSI platforms in order to gain the maximum molecular information about a sample that would otherwise be limited by a single analytical technique. The aim of this ...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2021-10-11 | MSV000088211 | MassIVE
UnknownAutomated Coupling of Nanodroplet Sample Preparation with Liquid Chromatography-Mass Spectrometry for High-Throughput Single-Cell Proteomics.
Not available
S-EPMC7793572 | biostudies-literature
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