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ProteomicsSTIC2 selectively binds ribosome-nascent chain complexes in the cotranslational sorting of Arabidopsis thylakoid proteins
Chloroplast-encoded multi-span thylakoid membrane proteins are crucial for photosynthetic complexes, yet the coordination of their biogenesis remains poorly understood. To identify factors that specifically support the cotranslational biogenesis of the reaction center protein D1 of photosystem (PS) ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-28 | PXD042896 | Pride
Thylakoid membrane Photosystem ii Arabidopsis Cotranslational targeting Stic2 
ProteomicsAnalysis of stalled ribosome-nascent chain complexes by quantitative proteomics
The cellular environment is critical for efficient protein maturation, but how proteins fold during biogenesis remains poorly understood. To understand how the cellular environment modulates folding, we studied the cotranslational chaperone-assisted folding of Escherichia coli dihydrofolate reductas...
ORGANISM(S): Escherichia coli 
2025-05-06 | PXD036784 | Pride
Protein biosynthesis Ribosome-nascent chain complexes Interactome Protein folding Lc-ms/ms 
ProteomicsNascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
mRNAs are generally assumed to be loaded instantly with ribosomes upon entry into the cytoplasm. To measure ribosome density on nascent mRNA, we developed nascent Ribo-Seq (nRibo-Seq) by combining Ribo-Seq with progressive 4-thiouridine labelling. In mouse macrophages, we experimentally determined, ...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-22 | PXD026828 | Pride
Ribo-seq Translatome Nascent peptides 
UnknownTma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae.
Not available
S-EPMC5062994 | biostudies-literature
ProteomicsSECRETOME DATA only from “Selective stalling of human translation through small molecule engagement of the ribosome nascent chain”
Human genetics as well as pharmacological intervention reveal that Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) plays a key role in regulating the levels of plasma low density lipoprotein cholesterol (LDL-C). Here we demonstrate that the compound PF-06446846 inhibits translation of PCSK9 by...
ORGANISM(S): Homo sapiens (Human) 
2017-03-27 | PXD005822 | Pride
Translational stalling Cell lysate Silac Pcsk9 Huh7 cells Secretome 
ProteomicsCELL LYSATE DATA only from “Selective stalling of human translation through small molecule engagement of the ribosome nascent chain”
Human genetics as well as pharmacological intervention reveal that Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) plays a key role in regulating the levels of plasma low density lipoprotein cholesterol (LDL-C). Here we demonstrate that the compound PF-06446846 inhibits translation of PCSK9 by...
ORGANISM(S): Homo sapiens (Human) 
2017-03-27 | PXD005835 | Pride
Translational stalling Silac Pcsk9 Huh7 cells 
ProteomicsGlobal profiling of nascent chain interactors reveals TRIM25 as a novel co-translational E3 ubiquitin ligase
Nascent polypeptide chains emerging from the ribosome engage a range of co-translational factors at distinct phases of translation. These co-translational interactions are crucial for proper protein biogenesis and quality control pathways to maintain protein homeostasis. Hence, systematic identifica...
ORGANISM(S): Homo Sapiens (human) 
2026-03-19 | PXD062019 | JPOST Repository
UnknownA ribosome-associating chaperone mediates GTP-driven vectorial folding of nascent eEF1A.
Not available
S-EPMC11790920 | biostudies-literature
ProteomicsProteomic changes of tumor drug-resistant cells after drug treatment
Whole proteome and ribosome-nascent-chain complex (RNC) changes of A549 cisplatin-resistant cells after treated with aurintricarboxylic acid
ORGANISM(S): Homo Sapiens 
2025-02-20 | PXD061028 |
UnknownProtein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains.
Not available
S-EPMC4451101 | biostudies-literature
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