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TranscriptomicsNascent elongating transcript sequencing (NET-seq) for Escherichia coli and Bacillus subtilis reveals a consensus pause sequence enriched at translation start sites.
Transcription by RNA polymerase (RNAP) is interrupted by pauses that play diverse regulatory roles. Although individual pauses have been studied in vitro, the determinants of pauses in vivo and their distribution throughout the bacterial genome remain unknown. Using nascent transcript sequencing we ...
ORGANISM(S): Bacillus subtilis 
2014-05-05 | E-GEOD-56720 | biostudies-arrayexpress
ProteomicsIdentification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells
Accurate translation of mRNAs into functional proteins is a fundamental process in all living organisms. In bacteria, in the early stage of translation elongation, peptidyl-tRNAs (pep-tRNAs) with short nascent chains frequently dissociate from the ribosome (pep-tRNA drop-off). The dissociated pep-tR...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD030807 | Pride
Translation 
ProteomicsIdentification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells
Accurate translation of mRNAs into functional proteins is a fundamental process in all living organisms. In bacteria, in the early stage of translation elongation, peptidyl-tRNAs (pep-tRNAs) with short nascent chains frequently dissociate from the ribosome (pep-tRNA drop-off). The dissociated pep-tR...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD030808 | Pride
Translation 
ProteomicsIdentification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells
Accurate translation of mRNAs into functional proteins is a fundamental process in all living organisms. In bacteria, in the early stage of translation elongation, peptidyl-tRNAs (pep-tRNAs) with short nascent chains frequently dissociate from the ribosome (pep-tRNA drop-off). The dissociated pep-tR...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD030806 | Pride
Translation 
ProteomicsIdentification of nascent peptides of peptidyl-tRNA dissociated from ribosome in the early elongation stage in Escherichia coli pthts cells
Accurate translation of mRNAs into functional proteins is a fundamental process in all living organisms. In bacteria, in the early stage of translation elongation, peptidyl-tRNAs (pep-tRNAs) with short nascent chains frequently dissociate from the ribosome (pep-tRNA drop-off). The dissociated pep-tR...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD030805 | Pride
Translation 
ProteomicsA mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism
The mini-hairpin shaped nascent peptide that blocks translation termination was found by comprehensive proteomics approach for small ORFs overexpression.
ORGANISM(S): Escherichia Coli 
2025-02-17 | PXD052430 | JPOST Repository
ProteomicsNascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
mRNAs are generally assumed to be loaded instantly with ribosomes upon entry into the cytoplasm. To measure ribosome density on nascent mRNA, we developed nascent Ribo-Seq (nRibo-Seq) by combining Ribo-Seq with progressive 4-thiouridine labelling. In mouse macrophages, we experimentally determined, ...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-22 | PXD026828 | Pride
Ribo-seq Translatome Nascent peptides 
UnknownNuclear processing of nascent transcripts determines synthesis of full-length proteins and antigenic peptides.
Not available
S-EPMC6451098 | biostudies-literature
UnknownMacrolide-peptide conjugates as probes of the path of travel of the nascent peptides through the ribosome.
Not available
S-EPMC4245169 | biostudies-literature
ProteomicsTemporary specific alternations in the nascent proteome of HEK293T cells in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine
This dataset elucidates the remodeling of the nascent proteome before and upon global translation attenuation in response to transient mitochondrial depolarization by carbonyl cyanide m-chlorophenyl hydrazine (CCCP). During and following CCCP treatment, nascent proteome in short (30min) time windows...
ORGANISM(S): Homo sapiens (Human) 
2024-10-02 | PXD043942 | Pride
Boncat Protein translation Mitochondrial stress Nascent proteome 
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