Sort   by:  
 Page size 
ProteomicsBinding proteins for the GA-modified proteins in mouse serum.
We determined if the glycolaldehyde (GA)-modified proteins could show an affinity with serum proteins. The mouse serum was incubated with the ligand-coupled beads for 1 h at room temperature. The ligands used were BSA and GA-treated BSA (GA-BSA). The proteins bound to the beads were eluted and separ...
ORGANISM(S): Mus Musculus (mouse) 
2020-04-08 | PXD018423 | JPOST Repository
ProteomicsCrosslinking mass spectrometry of the p300 catalytic core complexed with the nucleosome
p300 is a histone acetyltransferase that associates with crucial biological processes. p300 acetylates all four histones in the nucleosome, a basic unit of chromatin, and alters chromatin structure and dynamics. In this study, we performed structural and biochemical analysis to understand the nucleo...
ORGANISM(S): Homo Sapiens (human) 
2022-06-29 | PXD033804 | JPOST Repository
ProteomicsPhosphorylation sites that influences the RNA-binding activity of Papi
We conducted UV cross-linking immunoprecipitation (CLIP) with and without phosphatase inhibitor and compared the outcome. The topmost band on the western blots appeared more prominent with the inhibitor. The RNA-binding activity of the band was also stronger with the inhibitor. We isolated the band ...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027395 | JPOST Repository
ProteomicsPapi interacting proteins in mitochondrial fraction of BmN4 cells
We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027396 | JPOST Repository
ProteomicsAgo3 interaction proteins in BmN4 cell
We prepared BmN4 cell lysates in Co-IP buffer [30 mM HEPES-KOH pH 7.3, 150 mM potassium acetate, 5 mM magnesium acetate, 5 mM dithiothreitol, 0.1% (w/v) NP-40 and cOmplete ULTRA EDTA-free protease inhibitor (Roche)] with and without 1 mM EDTA and conducted immunoprecipitation assays using anti-Ago3...
ORGANISM(S): Bombyx Mori 
2021-01-08 | PXD023467 | JPOST Repository
ProteomicsNucleosome binding by the pioneer transcription factor OCT4
The nucleosome is a fundamental unit of chromatin in eukaryotes, and generally prevents the binding of transcription factors to genomic DNA. Pioneer transcription factors overcome the nucleosome barrier, and bind their target DNA sequences in chromatin. OCT4 is a representative pioneer transcription...
ORGANISM(S): Homo Sapiens (human) 
2020-05-13 | PXD019160 | JPOST Repository
ProteomicsAcetylation of p73 in 293T cell
293T cells ectopically expressing FLAG-tagged p73 were treated with AGK2 and p73 was immunoprecipitated. The precipitants were resuspended in deacetylation buffer (50 mM Tris-HCl pH 8.0, 140 mM NaCl, 2 mM MgCl 2 ) and incubated with recombinant SIRT2 protein (10 U). The reaction was terminated by bo...
ORGANISM(S): Homo Sapiens (human) 
2018-09-10 | PXD010411 | JPOST Repository
Sort   by:  
 Page size