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ProteomicsLow glucose culture conditions alter neuronal energetics, mimicking in vivo studies
Neurons are almost exclusively cultured in media containing glucose at much higher concentration than found in the brain. To test whether “standard” hyperglycaemic culture conditions affect neuronal respiration compared to near- euglycemic conditions, we grew neuronal cultures with minimal glial con...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-30 | PXD059008 | Pride
Primary mouse neuron Cell culture Glucose Mitochondrial respiration Neurobasal media Neuronal bioenergetics 
TranscriptomicsDistinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts
Bulk RNA sequencing was performed on human glial progenitor cells (hGPCs) derived from the hESC line RC17 and reprogrammed toward a dopaminergic neuron fate using Ascl1, Lmx1a, Nurr1 and shREST (ALNRi). Cells were cultured under two media conditions; standard NDiff medium or a B27/Neurobasal-based m...
ORGANISM(S): Homo sapiens 
2026-08-21 | GSE333803 | GEO
ProteomicsSensory nerve-secreted factors regulate basal keratinocyte function in vitro
Injuries to the skin can result in non-healing wounds, characterized by prolonged inflammation, failure to close, and chronic pain. Basal keratinocytes in the epidermis respond to signals activated following injury by proliferating, migrating, and differentiating to restore the epidermal barrier. Th...
ORGANISM(S): Mus musculus (Mouse) 
2025-03-28 | PXD062182 | Pride
Wound healing Cell migration Cell proliferation Re-epithelialization Secretome 
GenomicsDistinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts
Distinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts
PRJNA1472565 | ENA
TranscriptomicsRNA-seq datasets for "Temporally Specific Gene Expression and Chromatin Remodeling Programs Regulate a Conserved Pdyn Enhancer" [RNA-seq]
This dataset contains RNA-seq results from rat embryonic striatal neuronal cultures treated with either vehicle (complete Neurobasal media) or potassium chloride (10mM) for 4 h.
ORGANISM(S): Rattus norvegicus 
2023-08-07 | GSE233752 | GEO
GenomicsRNA-seq datasets for "Temporally Specific Gene Expression and Chromatin Remodeling Programs Regulate a Conserved Pdyn Enhancer"
This dataset contains Assay for Transposase-Accessible Chromatin (ATAC) sequencing results from rat embryonic striatal neuronal cultures treated with either vehicle (complete Neurobasal media) or potassium chloride (10mM) for 4 h.
ORGANISM(S): Rattus norvegicus 
2023-08-07 | GSE233368 | GEO
GenomicsATAC-seq datasets for chromatin accessibility quantification in "Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems"
This dataset contains Assay for Transposase-Accessible Chromatin (ATAC) sequencing results from rat embryonic cortical, hippocampal, and striatal neuronal cultures treated with either vehicle (complete Neurobasal media) or potassium chloride (10mM) for 1hr.
ORGANISM(S): Rattus norvegicus 
2020-07-28 | GSE150589 | GEO
TranscriptomicsRNA-seq datasets for enhancer RNA quantification in "Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems"
This dataset contains total RNA sequencing results from rat embryonic cortical, hippocampal, and striatal neuronal cultures treated with either vehicle (complete Neurobasal media) or potassium chloride (10mM) for 1hr prior to RNA extraction.
ORGANISM(S): Rattus norvegicus 
2020-07-28 | GSE150499 | GEO
TranscriptomicsRapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors
Here, we report synergistic inhibition of glycogen synthase kinase 3 (GSK3), transforming growth factor β (TGF β), and Notch signaling pathways by small molecules can efficiently convert monolayer cultured hESCs into homogenous primitive neuroepithelium within one week under chemically defined condi...
ORGANISM(S): Homo sapiens 
2011-04-28 | E-GEOD-28595 | biostudies-arrayexpress
UnknownInfluence of basal media composition on barrier fidelity within human pluripotent stem cell-derived blood-brain barrier models.
Not available
S-EPMC8688328 | biostudies-literature
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