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Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
TranscriptomicsVitamin d receptor-mediated stromal reprogramming suppresses pancreatitis and enhances pancreatic cancer therapy
The poor clinical outcome in pancreatic ductal adenocarcinoma (PDA) has been attributed to intrinsic resistance to chemotherapy and a growth-permissive tumor microenvironment. Quiescent pancreatic stellate cells (PSCs) are neuroendocrine, nestin-positive, lipid-accumulating cells whose homologues i...
ORGANISM(S): Mus musculus 
2014-10-03 | E-GEOD-43770 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human activated T lymphocytes to determine the effects of TX527, a hypocalcemic vitamin D analog
Hypocalcemic vitamin D analogs are appealing molecules to exploit the immunomodulatory actions of active vitamin D in vivo. The functional modulation of dendritic cells is regarded as the key mechanism underlying their ability to regulate T cell responses. In contrast, the direct actions of vitamin ...
ORGANISM(S): Homo sapiens 
2010-11-15 | E-GEOD-23984 | biostudies-arrayexpress
GenomicsSequencing Apoptotic DNA Breakpoints
DNA fragmentation is a well-recognized hallmark of apoptosis. However, the precise DNA sequences cleaved during apoptosis triggered by distinct mechanisms remain unclear. We used next-generation sequencing of DNA fragments generated in Actinomycin D-treated human HL-60 leukemic cells to generate a h...
ORGANISM(S): Homo sapiens 
2011-10-15 | E-GEOD-32997 | biostudies-arrayexpress
TranscriptomicsmRNA-Seq of head tissue from Drosophila mojavensis
We sequenced mRNA from head tissue of females and male of Drosophila mojavensis to identify genes differentially expressed between the sexes and sex-specific alternative splicing events. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pa...
ORGANISM(S): Drosophila mojavensis 
2010-04-11 | E-GEOD-20882 | biostudies-arrayexpress
TranscriptomicsLow-dose actinomycin D preferentially disrupts EWS-FLI1DNA binding.
Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds that disrupt the binding of EWS-FLI1 to its cognate DNA targets. A number of DNA...
ORGANISM(S): Homo sapiens 
2013-08-26 | E-GEOD-45414 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human bronchial epithelial cells stimulated with the commensal bacterium S. salivarius K12 of the pathogens S. aureus, P. aeruginosa, or S. enteritidis
Human bronchial epithelial cells (16HBE14O-) were stimulated with the commensal bacterium S. salivarius K12, or the pathogens S. aureus, P. aeruginosa, or S. enteritidis (subtype Typhimurium) for 1 hour.
ORGANISM(S): Homo sapiens 
2008-10-30 | E-FPMI-13 | biostudies-arrayexpress
TranscriptomicsResistant starch induces catabolic but suppresses immune and cell division pathways and changes the microbiome in proximal colon of male pigs
Consumption of resistant starch (RS) has been associated with various intestinal health benefits, but knowledge on its effects on global gene expression in the colon is limited. The main objective of the current study was to identify genes affected by RS in the proximal colon to infer which biologic...
ORGANISM(S): Sus scrofa 
2013-10-18 | E-GEOD-45554 | biostudies-arrayexpress
TranscriptomicsVitamin D modulation of innate immune signalling in Dendritic Cells
Vitamin D is widely reported to inhibit innate immune signalling and dendritic cell (DC) maturation, leading to attenuation of DC mediated T cell activation as a potential immunoregulatory mechanism to guard against autoimmunity and immmunopathology. It is not known whether vitamin D has global or g...
ORGANISM(S): Homo sapiens 
2016-10-03 | E-MTAB-4883 | biostudies-arrayexpress
TranscriptomicsDevelopment of a Fluorescence-Activated Cell Sorting Method Coupled with Whole Genome Amplification To Analyze Minority and Trace Dehalococcoides Genomes in Microbial Communities
Dehalococcoides mccartyi are functionally important bacteria that catalyze the reductive dechlorination of chlorinated ethenes. However, these anaerobic bacteria are fastidious to isolate, making downstream genomic characterization challenging. In order to facilitate genomic analysis, a fluorescence...
ORGANISM(S): Dehalococcoides mccartyi 195 
2015-01-31 | E-GEOD-54040 | biostudies-arrayexpress
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