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TranscriptomicsGlobal expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained MEK/ERK and AP-1 activation during PC12 differentiation.
Neuronal differentiation of PC12 cells in response to NGF is a prototypical model in which signal duration determines a biological response. Sustained ERK activity induced by NGF, as compared to transient activity induced by EGF, is critical to the differentiation of these cells. To characterize the...
ORGANISM(S): Rattus norvegicus 
2011-11-13 | E-GEOD-33639 | biostudies-arrayexpress
ProteomicsProteomics of NGF-TrkA signaling dynamics in neuroblastoma cells
We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced TrkA signaling dynamics at the interactome, phosphoproteome and proteome level. A tetracycline-inducible system for TrkA expression was generated in the human neuroblastoma cell line, SH-SY5Y....
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080834 | MassIVE
TranscriptomicsSAGE libraries from cell bodies and axons of NGF-differentiated sympathetic neurons
The subcellular localization of specific mRNAs is an evolutionary conserved mechanism that underlies the establishment of cellular polarity and specialized cell functions. In neurons, mRNA trafficking and local protein translation in dendrites provides an important mechanism that mediates synaptic ...
ORGANISM(S): Rattus norvegicus 
2010-01-30 | E-GEOD-13788 | biostudies-arrayexpress
TranscriptomicsMicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation
MicroRNAs (miRNAs) are small non-coding RNAs that control protein expression through translational inhibition or mRNA degradation. MiRNAs have been implicated in diverse biological processes such as development, proliferation, apoptosis and differentiation. Upon treatment with nerve growth factor (N...
ORGANISM(S): Rattus norvegicus 
2012-05-15 | E-GEOD-32122 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death
Developing sympathetic neurons depend on nerve growth factor (NGF) for survival and die by apoptosis after NGF withdrawal. This process requires de novo gene expression but only a small number of genes induced by NGF deprivation have been identified so far. We have used Affymetrix Exon arrays to stu...
ORGANISM(S): Rattus norvegicus 
2013-01-17 | E-MTAB-696 | biostudies-arrayexpress
TranscriptomicsAnalysis of human neurotrophins and receptors in bone marrow stromal cells (BMSC) treated with NGF or BDNF
BMSC gene expression profiling was completed to examine the baseline neurotrophin and receptor gene expression in BMSC and to evaulate changes in gene expression in response to NGF or BDNF stimulation In the study, primary BMSC were treated with recombinant human NGF or BDNF for 24 hours. Following...
ORGANISM(S): Homo sapiens 
2010-05-01 | E-GEOD-18537 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of the AD11 anti-NGF transgenic mouse model of neurodegeneration at 1, 3, 6, 15 months of age
We characterized the gene expression profile of brain regions at different stages of the AlzheimerM-bM-^@M-^Ys like neurodegeneration in the anti-NGF AD11 trangenic mouse model. Total RNA was extracted from hippocampus, cortex and basal forebrain of postnatal day 30 (P30, 1 month), P90 (3 months), P...
ORGANISM(S): Mus musculus 
2015-02-26 | E-GEOD-63617 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile in PC12 cells under NGF treatment and deprivation
miRNA profiling of PC12 rat pheochromocytoma cells during NGF-induced differentiation and apoptosis of differentiated cells after 24h of NGF deprivation. miRNA expression in differentiating and deprivated cells was compared to untreated and terminally differentiated PC12 cells respectivelly. Five-c...
ORGANISM(S): Rattus norvegicus 
2013-05-11 | E-GEOD-46827 | biostudies-arrayexpress
TranscriptomicsTranscriptional programs controlled by NGF signaling in developing mouse dorsal root ganglia neurons
During embryogenesis, nociceptive sensory neurons of the dorsal root ganglia depend intimately on Nerve Growth Factor (NGF) for neuronal survival, maturation and target innervation. NGF is a secreted molecular signal synthesized by neuronal target tissues. In developing nociceptors, NGF engages th...
ORGANISM(S): Mus musculus 
2013-11-01 | E-GEOD-25611 | biostudies-arrayexpress
TranscriptomicsNGF and proNGF activate functionally distinct mRNAs in PC12 cells: an early gene expression profiling
The biological activities of NGF and of its precursor proNGF are quite distinct, due to different receptor binding profiles, but nothing is known about how proNGF regulates gene expression. We performed experiments to address this question, by verifying whether a proNGF specific transcriptional sign...
ORGANISM(S): Rattus norvegicus 
2012-01-09 | E-GEOD-26528 | biostudies-arrayexpress
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