Global analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death

ABSTRACT: Developing sympathetic neurons depend on nerve growth factor (NGF) for survival and die by apoptosis after NGF withdrawal. This process requires de novo gene expression but only a small number of genes induced by NGF deprivation have been identified so far. We have used Affymetrix Exon arrays to study the pattern of expression of all known genes in sympathetic neurons deprived of NGF. We identified 415 up- and 813 down-regulated genes, including most of the genes previously known to be regulated in this system. By including a mixed lineage kinase (MLK) inhibitor, CEP-11004, in our experimental design we identified which of the genes induced after NGF withdrawal are potential targets of the MLK-JNK-c-Jun pathway. A detailed Gene Ontology and functional enrichment analysis also identified ge

ORGANISM(S): Rattus norvegicus

SUBMITTER: Mark Kristiansen 

PROVIDER: E-MTAB-696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress