Excisable DD construct was fused with TurboID and transduced into NIH3T3 cells. The cells were exposed to Shield-1 (1 μM) for 24 h, followed by co-treatment of Shield-1 (1 μM) and biotin (50 µM) for 24 h (biological replicates; n = 3). The cell lysates in guanidine-TCEP buffer were dissolved by heat...
ORGANISM(S): Mus Musculus (mouse)