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TranscriptomicsAnalysis of transgene siRNAs and ARL-8-dependent siRNAs in Caenorhabditis elegans
We analyzed the C. elegans small RNA response to high copy transgene sequences expressed in the soma in a wild type and an eri-6/7 mutant background. We also analyzed small RNA defects in the arl-8(tm2472) mutant. Transgene siRNAs are 22 nt long, mostly antisense, and correspond to the promoter, cod...
ORGANISM(S): Caenorhabditis elegans 
2013-12-15 | E-GEOD-52785 | biostudies-arrayexpress
Transcriptomics26G endo-siRNAs regulate spermatogenic and zygotic gene expression
Analysis of small RNAs with 5'p, 3'OH Small RNAs (18-32nt) expressed in purified male sperm, hermaphrodite oocytes, embryos, N2, glp-4(bn2), and eri-1(mg366) were cloned and sequenced by 454 and Illumina high-throughput sequencing.
ORGANISM(S): Caenorhabditis elegans 
2010-05-24 | E-GEOD-20341 | biostudies-arrayexpress
TranscriptomicsSmall RNA profiling of wildtype and Eri1-deficient mouse T cells
Natural killer (NK) cells play a critical role in early host defense to infected and transformed cells. Here we show that mice deficient in Eri1, a conserved 3M-bM-^@M-^Y-to-5M-bM-^@M-^Y exoribonuclease that represses RNA interference, have a cell-intrinsic defect in NK cell development and maturati...
ORGANISM(S): Mus musculus 
2012-07-11 | E-GEOD-31920 | biostudies-arrayexpress
UnknownRyR2R420Q catecholaminergic polymorphic ventricular tachycardia mutation induces bradycardia by disturbing the coupled clock pacemaker mechanism.
Not available
S-EPMC5396513 | biostudies-literature
TranscriptomicsFLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)
piRNAs function in silencing retrotransposons by associating with the PIWI proteins, AGO3, Aub, and Piwi, in Drosophila germlines. Bioinformatics analyses of piRNAs in Drosophila ovaries suggested that piRNAs are produced by two systems, the primary processing pathway and the amplification loop, fro...
ORGANISM(S): Drosophila melanogaster 
2009-10-01 | E-GEOD-15137 | biostudies-arrayexpress
ProteomicsProximity biotinylation of iNKT cells expressing TurboID-Ikaros with or without TCR stimulation
Ikaros cDNA fused to TurboID was cloned into a retroviral vector and transfected into Phoenix retrovirus packaging cells using PEI MAX (Polysceinces). Culture supernatant containing retroviruses was used for infection into DN32.D3 cells. Cells were cultured in 10%FBS/DMEM which does not contain biot...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059289 | JPOST Repository
ProteomicsTMT-based phosphoproteomic analysis of WT and PKD2/3-deficient iNKT cells
Parental and PKD2/3 double-deficient DN32.D3 cells were treated with 10 ug/ml anti-CD3 and cross-linked with 100 ug/ml goat anti-Hamster IgG for 5 min. Cells were washed with ice-cold HEPES-saline and lysed with Guanidine-HCl buffer (6 M guanidine-HCl, 100 mM Tris-Cl pH8.0, 2 mM DTT). The lysates we...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059288 | JPOST Repository
TranscriptomicsGene expression study identifies important protective pathways utilized by appendicitis and appendectomy for ameliorating colitis
BACKGROUND: Appendicitis followed by appendectomy (AA) at a young age protects against later inflammatory bowel disease (IBD). Using a novel murine appendicitis model we earlier demonstrated that AA proffered significant protection against subsequent experimental colitis. AIM: To delineate genes and...
ORGANISM(S): Mus musculus 
2010-09-01 | E-GEOD-23914 | biostudies-arrayexpress
UnknownProtein Kinase D2 drives chylomicron-mediated lipid transport in the intestine and promotes obesity
Not available
S-SCDT-EMM-2020-13548 | biostudies-other
TranscriptomicsTranscription profiling of mouse intestine in ENU mutagenesis mouse strains with missense mutations in Muc2 mucin and ER stress
Background; MUC2 mucin produced by intestinal goblet cells is the major component of the intestinal mucus barrier. MUC2 homo-oligomerizes intracellularly into large secreted polymers which give mucus its viscous properties. The inflammatory bowel disease (IBD) ulcerative colitis is characterized by...
ORGANISM(S): Mus musculus 
2008-04-06 | E-GEOD-9913 | biostudies-arrayexpress
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